Research data supporting "Phenotypic whole-cell screening identifies a protective carbohydrate epitope on Klebsiella pneumoniae"
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Berry, S. (2021). Research data supporting "Phenotypic whole-cell screening identifies a protective carbohydrate epitope on Klebsiella pneumoniae" [Dataset]. https://doi.org/10.17863/CAM.78423
Summary: The data set contains genome sequences of Klebsiella pneumoniae strains from a global panel of clinical isolates. The IHMA isolates were collected in in 2012 from hospitalised patients as part of a larger collection from an international antibiotic resistance surveillance programme. The metadata file contains information about the isolates. Abbreviations: GU, genitourinary; GI, gastrointestinal; INT, integument. Clinical isolates were obtained from the International Health Management Associates (IHMA) as part of a collection from an international antibiotic resistance surveillance program. Basic demographic data (age, sex, hospital location, sample type, and length of stay) were provided for each isolate by the IHMA using a unique study number that was delinked from any patient identification. DNA was purified from bacterial cultures via bead beating followed by extraction using a PureLink Genomic DNA Mini Kit (ThermoFisher). Sequencing libraries were prepared by mechanical shearing of DNA (Covaris) followed by a NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs). Libraries were then run on an Illumina HiSeq, 2x150bp configuration. Sequence data was quality-trimmed, de-novo assembled and annotated using BugBuilder.
The assembled genome files can be viewed using a text editor, or using DNA analysis software, for example Snapgene.
klesiella pneumoniae, genome
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This record's DOI: https://doi.org/10.17863/CAM.78423
Attribution 4.0 International (CC BY 4.0)
Licence URL: https://creativecommons.org/licenses/by/4.0/