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dc.contributor.authorMorgan, Chris
dc.contributor.authorFozard, John A
dc.contributor.authorHartley, Matthew
dc.contributor.authorHenderson, Ian
dc.contributor.authorBomblies, Kirsten
dc.contributor.authorHoward, Martin
dc.date.accessioned2021-11-25T00:30:33Z
dc.date.available2021-11-25T00:30:33Z
dc.date.issued2021-08-03
dc.identifier.issn2041-1723
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/331058
dc.description.abstractIn most organisms, the number and distribution of crossovers that occur during meiosis are tightly controlled. All chromosomes must receive at least one 'obligatory crossover' and crossovers are prevented from occurring near one another by 'crossover interference'. However, the mechanistic basis of this phenomenon of crossover interference has remained mostly mysterious. Using quantitative super-resolution cytogenetics and mathematical modelling, we investigate crossover positioning in the Arabidopsis thaliana wild-type, an over-expressor of the conserved E3 ligase HEI10, and a hei10 heterozygous line. We show that crossover positions can be explained by a predictive, diffusion-mediated coarsening model, in which large, approximately evenly-spaced HEI10 foci grow at the expense of smaller, closely-spaced clusters. We propose this coarsening process explains many aspects of Arabidopsis crossover positioning, including crossover interference. Consistent with this model, we also demonstrate that crossover positioning can be predictably modified in vivo simply by altering HEI10 dosage, with higher and lower dosage leading to weaker and stronger crossover interference, respectively. As HEI10 is a conserved member of the RING finger protein family that functions in the interference-sensitive pathway for crossover formation, we anticipate that similar mechanisms may regulate crossover positioning in diverse eukaryotes.
dc.format.mediumElectronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectChromosomes, Plant
dc.subjectSynaptonemal Complex
dc.subjectArabidopsis
dc.subjectChromosomal Proteins, Non-Histone
dc.subjectArabidopsis Proteins
dc.subjectPachytene Stage
dc.subjectMeiosis
dc.subjectCrossing Over, Genetic
dc.subjectGene Dosage
dc.subjectComputer Simulation
dc.titleDiffusion-mediated HEI10 coarsening can explain meiotic crossover positioning in Arabidopsis.
dc.typeArticle
prism.issueIdentifier1
prism.publicationDate2021
prism.publicationNameNat Commun
prism.startingPage4674
prism.volume12
dc.identifier.doi10.17863/CAM.78503
dcterms.dateAccepted2021-07-12
rioxxterms.versionofrecord10.1038/s41467-021-24827-w
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-08-03
dc.contributor.orcidMorgan, Chris [0000-0002-7475-2155]
dc.contributor.orcidFozard, John A [0000-0001-9181-8083]
dc.contributor.orcidHenderson, Ian [0000-0001-5066-1489]
dc.contributor.orcidHoward, Martin [0000-0001-7670-0781]
dc.identifier.eissn2041-1723
rioxxterms.typeJournal Article/Review
pubs.funder-project-idLeverhulme Trust (RPG-2019-259)
cam.issuedOnline2021-08-03


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International