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dc.contributor.authorSechi, Stefano
dc.contributor.authorKarimpour-Ghahnavieh, Angela
dc.contributor.authorFrappaolo, Anna
dc.contributor.authorDi Francesco, Laura
dc.contributor.authorPiergentili, Roberto
dc.contributor.authorSchininà, Eugenia
dc.contributor.authorD'Avino, Paolo
dc.contributor.authorGiansanti, Maria Grazia
dc.date.accessioned2021-11-25T17:29:11Z
dc.date.available2021-11-25T17:29:11Z
dc.date.issued2021-09-06
dc.identifier.issn2073-4409
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/331188
dc.description.abstractGolgi phosphoprotein 3 (GOLPH3) is a highly conserved peripheral membrane protein localized to the Golgi apparatus and the cytosol. GOLPH3 binding to Golgi membranes depends on phosphatidylinositol 4-phosphate [PI(4)P] and regulates Golgi architecture and vesicle trafficking. GOLPH3 overexpression has been correlated with poor prognosis in several cancers, but the molecular mechanisms that link GOLPH3 to malignant transformation are poorly understood. We recently showed that PI(4)P-GOLPH3 couples membrane trafficking with contractile ring assembly during cytokinesis in dividing Drosophila spermatocytes. Here, we use affinity purification coupled with mass spectrometry (AP-MS) to identify the protein-protein interaction network (interactome) of Drosophila GOLPH3 in testes. Analysis of the GOLPH3 interactome revealed enrichment for proteins involved in vesicle-mediated trafficking, cell proliferation and cytoskeleton dynamics. In particular, we found that dGOLPH3 interacts with the Drosophila orthologs of Fragile X mental retardation protein and Ataxin-2, suggesting a potential role in the pathophysiology of disorders of the nervous system. Our findings suggest novel molecular targets associated with GOLPH3 that might be relevant for therapeutic intervention in cancers and other human diseases.
dc.format.mediumElectronic
dc.languageeng
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleIdentification of GOLPH3 Partners in Drosophila Unveils Potential Novel Roles in Tumorigenesis and Neural Disorders.
dc.typeArticle
prism.issueIdentifier9
prism.publicationDate2021
prism.publicationNameCells
prism.volume10
dc.identifier.doi10.17863/CAM.78635
dc.identifier.doi10.17863/CAM.78635
dcterms.dateAccepted2021-09-03
rioxxterms.versionofrecord10.3390/cells10092336
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-09-06
dc.contributor.orcidFrappaolo, Anna [0000-0002-3270-4139]
dc.contributor.orcidPiergentili, Roberto [0000-0001-7584-2171]
dc.contributor.orcidD'Avino, Paolo [0000-0002-4773-6950]
dc.contributor.orcidGiansanti, Maria Grazia [0000-0002-6753-7262]
dc.identifier.eissn2073-4409
rioxxterms.typeJournal Article/Review
cam.issuedOnline2021-09-06


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International