Show simple item record

dc.contributor.authorZyner, Katherine G
dc.contributor.authorSimeone, Angela
dc.contributor.authorFlynn, Sean M
dc.contributor.authorDoyle, Colm
dc.contributor.authorMarsico, Giovanni
dc.contributor.authorAdhikari, Santosh
dc.contributor.authorPortella, Guillem
dc.contributor.authorTannahill, David
dc.contributor.authorBalasubramanian, Shankar
dc.date.accessioned2021-12-04T00:30:29Z
dc.date.available2021-12-04T00:30:29Z
dc.date.issued2022-01-10
dc.identifier.issn2041-1723
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/331230
dc.description.abstractThe establishment of cell identity during embryonic development involves the activation of specific gene expression programmes and is underpinned by epigenetic factors including DNA methylation and histone post-translational modifications. G-quadruplexes are four-stranded DNA secondary structures (G4s) that have been implicated in transcriptional regulation and cancer. Here, we show that G4s are key genomic structural features linked to cellular differentiation. We find that G4s are highly abundant in human embryonic stem cells and are lost during lineage specification. G4s are prevalent in enhancers and promoters. G4s that are found in common between embryonic and downstream lineages are tightly linked to transcriptional stabilisation of genes involved in essential cellular functions as well as transitions in the histone post-translational modification landscape. Furthermore, the application of small molecules that stabilise G4s causes a delay in stem cell differentiation, keeping cells in a more pluripotent-like state. Collectively, our data highlight G4s as important epigenetic features that are coupled to stem cell pluripotency and differentiation.
dc.publisherNature Research
dc.rightsAll rights reserved
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserved
dc.titleG-quadruplex DNA structures in human stem cells and differentiation.
dc.typeArticle
dc.publisher.departmentoffice of The School of Clinical Medicine
dc.date.updated2021-12-03T12:20:56Z
prism.publicationNameNat Commun
dc.identifier.doi10.17863/CAM.78676
dcterms.dateAccepted2021-12-02
rioxxterms.versionofrecord10.1038/s41467-021-27719-1
rioxxterms.versionAM
dc.contributor.orcidZyner, Katherine G [0000-0002-4997-0150]
dc.contributor.orcidFlynn, Sean M [0000-0001-7326-2659]
dc.contributor.orcidAdhikari, Santosh [0000-0002-1501-2106]
dc.contributor.orcidTannahill, David [0000-0002-3811-6864]
dc.contributor.orcidBalasubramanian, Shankar [0000-0002-0281-5815]
dc.identifier.eissn2041-1723
rioxxterms.typeJournal Article/Review
pubs.funder-project-idCancer Research UK (CB4330)
pubs.funder-project-idCancer Research UK (C9681/A29214)
pubs.funder-project-idWellcome Trust (209441/Z/17/Z)
cam.orpheus.successTue Feb 01 19:02:22 GMT 2022 - Embargo updated*
cam.depositDate2021-12-03
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement
rioxxterms.freetoread.startdate2022-01-10


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record