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dc.contributor.authorGaunt, Adam P
dc.contributor.authorLewis, Jennifer S
dc.contributor.authorHesse, Friederike
dc.contributor.authorCheng, Tian
dc.contributor.authorMarco-Rius, Irene
dc.contributor.authorBrindle, Kevin
dc.contributor.authorComment, Arnaud
dc.date.accessioned2021-12-15T10:06:24Z
dc.date.available2021-12-15T10:06:24Z
dc.date.issued2022-01-10
dc.date.submitted2021-09-23
dc.identifier.issn1433-7851
dc.identifier.otheranie202112982
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/331418
dc.description.abstractHyperpolarized (HP) 13 C magnetic resonance enables non-invasive probing of metabolism in vivo. To date, only 13 C-molecules hyperpolarized with persistent trityl radicals have been injected in humans. We show here that the free radical photo-induced in alpha-ketoglutaric acid (α-KG) can be used to hyperpolarize photo-inactive 13 C-molecules such as [1-13 C]lactate. α-KG is an endogenous molecule with an exceptionally high radical yield under photo-irradiation, up to 50 %, and its breakdown product, succinic acid, is also endogenous. This radical precursor therefore exhibits an excellent safety profile for translation to human studies. The labile nature of the radical means that no filtration is required prior to injection while also offering the opportunity to extend the 13 C relaxation time in frozen HP 13 C-molecules for storage and transport. The potential for in vivo metabolic studies is demonstrated in the rat liver following the injection of a physiological dose of HP [1-13 C]lactate.
dc.languageen
dc.publisherWiley
dc.subjectResearch Article
dc.subjectResearch Articles
dc.subjectdynamic nuclear polarization
dc.subjectimaging agents
dc.subjectketo acids
dc.subjectmetabolism
dc.subjectphotochemistry
dc.titleLabile Photo-Induced Free Radical in α-Ketoglutaric Acid: a Universal Endogenous Polarizing Agent for In Vivo Hyperpolarized 13 C Magnetic Resonance.
dc.typeArticle
dc.date.updated2021-12-15T10:06:22Z
prism.publicationNameAngew Chem Int Ed Engl
dc.identifier.doi10.17863/CAM.78872
rioxxterms.versionofrecord10.1002/anie.202112982
rioxxterms.versionAO
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidBrindle, Kevin [0000-0003-3883-6287]
dc.contributor.orcidComment, Arnaud [0000-0002-8484-3448]
dc.identifier.eissn1521-3773
pubs.funder-project-idCancer Research UK (CB4100)
pubs.funder-project-idCancer Research UK (C14303/A17197)
pubs.funder-project-idEuropean Research Council (682574)
pubs.funder-project-idEuropean Commission Horizon 2020 (H2020) ERC (963998)
cam.issuedOnline2021-11-25


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