Labile Photo‐Induced Free Radical in α‐Ketoglutaric Acid: a Universal Endogenous Polarizing Agent for In Vivo Hyperpolarized <sup>13</sup> C Magnetic Resonance
Authors
Gaunt, Adam P
Lewis, Jennifer S
Hesse, Friederike
Cheng, Tian
Marco‐Rius, Irene
Publication Date
2022-01-10Journal Title
Angewandte Chemie
ISSN
0044-8249
Publisher
Wiley
Language
en
Type
Article
This Version
AO
VoR
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Gaunt, A. P., Lewis, J. S., Hesse, F., Cheng, T., Marco‐Rius, I., Brindle, K., & Comment, A. (2022). Labile Photo‐Induced Free Radical in α‐Ketoglutaric Acid: a Universal Endogenous Polarizing Agent for In Vivo Hyperpolarized
<sup>13</sup>
C Magnetic Resonance. Angewandte Chemie https://doi.org/10.1002/ange.202112982
Abstract
Abstract: Hyperpolarized (HP) 13C magnetic resonance enables non‐invasive probing of metabolism in vivo. To date, only 13C‐molecules hyperpolarized with persistent trityl radicals have been injected in humans. We show here that the free radical photo‐induced in alpha‐ketoglutaric acid (α‐KG) can be used to hyperpolarize photo‐inactive 13C‐molecules such as [1‐13C]lactate. α‐KG is an endogenous molecule with an exceptionally high radical yield under photo‐irradiation, up to 50 %, and its breakdown product, succinic acid, is also endogenous. This radical precursor therefore exhibits an excellent safety profile for translation to human studies. The labile nature of the radical means that no filtration is required prior to injection while also offering the opportunity to extend the 13C relaxation time in frozen HP 13C‐molecules for storage and transport. The potential for in vivo metabolic studies is demonstrated in the rat liver following the injection of a physiological dose of HP [1‐13C]lactate.
Keywords
Forschungsartikel, dynamic nuclear polarization, imaging agents, keto acids, metabolism, photochemistry
Sponsorship
Cancer Research UK (CB4100)
Cancer Research UK (C14303/A17197)
European Commission Horizon 2020 (H2020) ERC (963998)
European Research Council (682574)
Identifiers
ange202112982
External DOI: https://doi.org/10.1002/ange.202112982
This record's URL: https://www.repository.cam.ac.uk/handle/1810/331419
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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