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dc.contributor.authorStangherlin, Alessandra
dc.contributor.authorWatson, Joseph L
dc.contributor.authorWong, David CS
dc.contributor.authorBarbiero, Silvia
dc.contributor.authorZeng, Aiwei
dc.contributor.authorSeinkmane, Estere
dc.contributor.authorChew, Sew Peak
dc.contributor.authorBeale, Andrew D
dc.contributor.authorHayter, Edward A
dc.contributor.authorGuna, Alina
dc.contributor.authorInglis, Alison J
dc.contributor.authorPutker, Marrit
dc.contributor.authorBartolami, Eline
dc.contributor.authorMatile, Stefan
dc.contributor.authorLequeux, Nicolas
dc.contributor.authorPons, Thomas
dc.contributor.authorDay, Jason
dc.contributor.authorvan Ooijen, Gerben
dc.contributor.authorVoorhees, Rebecca M
dc.contributor.authorBechtold, David A
dc.contributor.authorDerivery, Emmanuel
dc.contributor.authorEdgar, Rachel S
dc.contributor.authorNewham, Peter
dc.contributor.authorO'Neill, John S
dc.date.accessioned2021-12-15T10:07:30Z
dc.date.available2021-12-15T10:07:30Z
dc.date.issued2021-10-15
dc.date.submitted2020-05-26
dc.identifier.issn2041-1723
dc.identifier.others41467-021-25942-4
dc.identifier.other25942
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/331427
dc.description.abstractBetween 6-20% of the cellular proteome is under circadian control and tunes mammalian cell function with daily environmental cycles. For cell viability, and to maintain volume within narrow limits, the daily variation in osmotic potential exerted by changes in the soluble proteome must be counterbalanced. The mechanisms and consequences of this osmotic compensation have not been investigated before. In cultured cells and in tissue we find that compensation involves electroneutral active transport of Na+, K+, and Cl- through differential activity of SLC12A family cotransporters. In cardiomyocytes ex vivo and in vivo, compensatory ion fluxes confer daily variation in electrical activity. Perturbation of soluble protein abundance has commensurate effects on ion composition and cellular function across the circadian cycle. Thus, circadian regulation of the proteome impacts ion homeostasis with substantial consequences for the physiology of electrically active cells such as cardiomyocytes.
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.subjectArticle
dc.subject/631/45/612/1248
dc.subject/631/80/105
dc.subject/631/443/592
dc.subject/96/106
dc.subject/14
dc.subject/14/19
dc.subject/82/58
dc.subject/64/60
dc.subject/9/30
dc.subjectarticle
dc.titleCompensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology.
dc.typeArticle
dc.date.updated2021-12-15T10:07:28Z
prism.issueIdentifier1
prism.publicationNameNat Commun
prism.volume12
dc.identifier.doi10.17863/CAM.78881
dcterms.dateAccepted2021-08-04
rioxxterms.versionofrecord10.1038/s41467-021-25942-4
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidStangherlin, Alessandra [0000-0001-7296-1183]
dc.contributor.orcidWatson, Joseph L [0000-0001-5492-0249]
dc.contributor.orcidWong, David CS [0000-0002-1712-9527]
dc.contributor.orcidBarbiero, Silvia [0000-0001-7177-6947]
dc.contributor.orcidZeng, Aiwei [0000-0003-0354-2529]
dc.contributor.orcidSeinkmane, Estere [0000-0002-3636-4709]
dc.contributor.orcidChew, Sew Peak [0000-0002-7602-6384]
dc.contributor.orcidBeale, Andrew D [0000-0002-2051-0919]
dc.contributor.orcidHayter, Edward A [0000-0001-9996-7198]
dc.contributor.orcidPons, Thomas [0000-0001-8800-4302]
dc.contributor.orcidDay, Jason [0000-0002-3078-0963]
dc.contributor.orcidvan Ooijen, Gerben [0000-0001-7967-0637]
dc.contributor.orcidBechtold, David A [0000-0001-8676-8704]
dc.contributor.orcidDerivery, Emmanuel [0000-0003-3927-5944]
dc.contributor.orcidNewham, Peter [0000-0002-0955-4080]
dc.contributor.orcidO'Neill, John S [0000-0003-2204-6096]
dc.identifier.eissn2041-1723
pubs.funder-project-idRCUK | Medical Research Council (MRC) (MC_UP_1201/4)
cam.issuedOnline2021-10-15


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