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dc.contributor.authorCharrier, Bénédicte
dc.contributor.authorBoscq, Samuel
dc.contributor.authorNelson, Bradley J
dc.contributor.authorLäubli, Nino F
dc.date.accessioned2021-12-15T12:12:21Z
dc.date.available2021-12-15T12:12:21Z
dc.date.issued2021-11-15
dc.date.submitted2021-07-22
dc.identifier.issn2296-7745
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/331485
dc.description.abstract<jats:p>Polydimethylsiloxane (PDMS) chips have proven to be suitable environments for the growth of several filamentous organisms. However, depending on the specimen, the number of investigations concerning their growth and cell differentiation is limited. In this work, we monitored the developmental pattern of the brown alga <jats:italic>Ectocarpus</jats:italic> inside PDMS lab-on-chips. Two main methods of inoculation of the lab-on-chip were tested, i.e., <jats:italic>via</jats:italic> the direct injection of spores into the chamber as well as through the insertion of sporophyte filaments. The resulting growth rate, growth trajectory, cell differentiation, and cell branching were monitored and quantified for 20 days inside 25 or 40 μm parallel channels under standard light and temperature conditions. With growth rates of 2.8 μm⋅h<jats:sup>–1</jats:sup>, normal growth trajectories and cell differentiation, as well as branching occurring inside the microfluidic environment, the main development steps were shown to be similar to those observed in non-constrained <jats:italic>in vitro</jats:italic> conditions. Additionally, the labelling of <jats:italic>Ectocarpus</jats:italic> cell wall polysaccharides using calcofluor for cellulose detection and immunolocalisation with monoclonal antibodies for alginates showed the expected patterns when compared to open space growth evaluated with either epifluorescence or confocal microscopy. Overall, this article describes the experimental conditions for observing and studying the basic unaltered processes of brown algal growth using microfluidic technology which provides the basis for future biochemical and biological researches.</jats:p>
dc.languageen
dc.publisherFrontiers Media SA
dc.subjectMarine Science
dc.subjectmicrofluidics
dc.subjectbrown alga
dc.subjecttip growth
dc.subjecton-chip immunolocalisation
dc.subjectEctocarpus
dc.subjectfilaments
dc.subjectlab-on-chips
dc.titleGrowth and Labelling of Cell Wall Components of the Brown Alga Ectocarpus in Microfluidic Chips
dc.typeArticle
dc.date.updated2021-12-15T12:12:20Z
prism.publicationNameFrontiers in Marine Science
prism.volume8
dc.identifier.doi10.17863/CAM.78939
dcterms.dateAccepted2021-10-25
rioxxterms.versionofrecord10.3389/fmars.2021.745654
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.identifier.eissn2296-7745
cam.issuedOnline2021-11-15


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