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dc.contributor.authorBaruch, Moshe
dc.contributor.authorTejedor-Sanz, Sara
dc.contributor.authorSu, Lin
dc.contributor.authorAjo-Franklin, Caroline M
dc.date.accessioned2021-12-18T00:30:57Z
dc.date.available2021-12-18T00:30:57Z
dc.date.issued2021
dc.identifier.issn1932-6203
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/331607
dc.description.abstractMicroorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we describe specific electrochemical control of the reduction of intracellular biomolecules in Escherichia coli through introduction of a heterologous electron transfer pathway. E. coli expressing cymAmtrCAB from Shewanella oneidensis MR-1 consumed electrons directly from a cathode when fumarate or nitrate, both intracellular electron acceptors, were present. The fumarate-triggered current consumption occurred only when fumarate reductase was present, indicating all the electrons passed through this enzyme. Moreover, CymAMtrCAB-expressing E. coli used current to stoichiometrically reduce nitrate. Thus, our work introduces a modular genetic tool to reduce a specific intracellular redox molecule with an electrode, opening the possibility of electronically controlling biological processes such as biosynthesis and growth in any microorganism.
dc.format.mediumElectronic-eCollection
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleElectronic control of redox reactions inside Escherichia coli using a genetic module.
dc.typeArticle
dc.publisher.departmentDepartment of Chemistry
dc.date.updated2021-12-16T16:13:05Z
prism.issueIdentifier11
prism.publicationDate2021
prism.publicationNamePLoS One
prism.startingPagee0258380
prism.volume16
dc.identifier.doi10.17863/CAM.79059
dcterms.dateAccepted2021-09-26
rioxxterms.versionofrecord10.1371/journal.pone.0258380
rioxxterms.versionVoR
dc.contributor.orcidTejedor-Sanz, Sara [0000-0002-1192-8256]
dc.contributor.orcidSu, Lin [0000-0001-8784-3120]
dc.identifier.eissn1932-6203
rioxxterms.typeJournal Article/Review
cam.issuedOnline2021-11-18
cam.depositDate2021-12-16
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International