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dc.contributor.authorTchasovnikarova, Iva A
dc.contributor.authorMarr, Sharon K
dc.contributor.authorDamle, Manashree
dc.contributor.authorKingston, Robert E
dc.date.accessioned2021-12-21T00:32:13Z
dc.date.available2021-12-21T00:32:13Z
dc.date.issued2022-01-20
dc.identifier.issn1097-2765
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/331649
dc.description.abstractGenetically encoded biosensors are powerful tools to monitor cellular behavior, but the difficulty in generating appropriate reporters for chromatin factors hampers our ability to dissect epigenetic pathways. Here, we present TRACE (transgene reporters across chromatin environments), a high-throughput, genome-wide technique to generate fluorescent human reporter cell lines responsive to manipulation of epigenetic factors. By profiling GFP expression from a large pool of individually barcoded lentiviral integrants in the presence and absence of a perturbation, we identify reporters responsive to pharmacological inhibition of the histone lysine demethylase LSD1 and genetic ablation of the PRC2 subunit SUZ12. Furthermore, by manipulating the HIV-1 host factor LEDGF through targeted deletion or fusion to chromatin reader domains, we alter lentiviral integration site preferences, thus broadening the types of chromatin examined by TRACE. The phenotypic reporters generated through TRACE will allow the genetic interrogation of a broad range of epigenetic pathways, furthering our mechanistic understanding of chromatin biology.
dc.description.sponsorshipDamon Runyon Cancer Research Foundation NIH
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleTRACE generates fluorescent human reporter cell lines to characterize epigenetic pathways.
dc.typeArticle
dc.publisher.departmentWellcome Trust/Cancer Research Uk Gurdon Institute
dc.date.updated2021-12-18T18:07:39Z
prism.publicationDate2022
prism.publicationNameMol Cell
dc.identifier.doi10.17863/CAM.79100
dcterms.dateAccepted2021-11-29
rioxxterms.versionofrecord10.1016/j.molcel.2021.11.035
rioxxterms.versionAM
dc.contributor.orcidTchasovnikarova, Iva [0000-0002-0477-0956]
dc.identifier.eissn1097-4164
rioxxterms.typeJournal Article/Review
pubs.funder-project-idDamon Runyon Cancer Research Foundation (42-20)
cam.issuedOnline2021-12-27
cam.orpheus.success2021-12-20 - Embargo set during processing via Fast-track
cam.depositDate2021-12-18
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement
rioxxterms.freetoread.startdate2022-12-27


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International