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dc.contributor.authorO'Neill, John
dc.contributor.authorHoyle, Nathaniel P
dc.contributor.authorRobertson, J Brian
dc.contributor.authorEdgar, Rachel S
dc.contributor.authorBeale, Andrew D
dc.contributor.authorPeak-Chew, Sew Y
dc.contributor.authorDay, Jason
dc.contributor.authorCosta, Ana SH
dc.contributor.authorFrezza, Christian
dc.contributor.authorCauston, Helen C
dc.date.accessioned2022-01-04T11:58:41Z
dc.date.available2022-01-04T11:58:41Z
dc.date.issued2020-09-17
dc.date.submitted2020-05-05
dc.identifier.issn2041-1723
dc.identifier.others41467-020-18330-x
dc.identifier.other18330
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/331847
dc.description.abstractYeast physiology is temporally regulated, this becomes apparent under nutrient-limited conditions and results in respiratory oscillations (YROs). YROs share features with circadian rhythms and interact with, but are independent of, the cell division cycle. Here, we show that YROs minimise energy expenditure by restricting protein synthesis until sufficient resources are stored, while maintaining osmotic homeostasis and protein quality control. Although nutrient supply is constant, cells sequester and store metabolic resources via increased transport, autophagy and biomolecular condensation. Replete stores trigger increased H+ export which stimulates TORC1 and liberates proteasomes, ribosomes, chaperones and metabolic enzymes from non-membrane bound compartments. This facilitates translational bursting, liquidation of storage carbohydrates, increased ATP turnover, and the export of osmolytes. We propose that dynamic regulation of ion transport and metabolic plasticity are required to maintain osmotic and protein homeostasis during remodelling of eukaryotic proteomes, and that bioenergetic constraints selected for temporal organisation that promotes oscillatory behaviour.
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.subjectArticle
dc.subject/631/45/320
dc.subject/631/45/475
dc.subject/631/1647/334/2243/1796
dc.subject/631/553/2701
dc.subject/49/31
dc.subject/49/47
dc.subject/96/35
dc.subject/82/58
dc.subjectarticle
dc.titleEukaryotic cell biology is temporally coordinated to support the energetic demands of protein homeostasis.
dc.typeArticle
dc.date.updated2022-01-04T11:58:40Z
prism.issueIdentifier1
prism.publicationNameNat Commun
prism.volume11
dc.identifier.doi10.17863/CAM.79297
dcterms.dateAccepted2020-08-13
rioxxterms.versionofrecord10.1038/s41467-020-18330-x
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidO'Neill, John [0000-0003-2204-6096]
dc.contributor.orcidHoyle, Nathaniel P [0000-0002-3250-0494]
dc.contributor.orcidBeale, Andrew D [0000-0002-2051-0919]
dc.contributor.orcidPeak-Chew, Sew Y [0000-0002-7602-6384]
dc.contributor.orcidCosta, Ana SH [0000-0001-8932-6370]
dc.contributor.orcidFrezza, Christian [0000-0002-3293-7397]
dc.identifier.eissn2041-1723
pubs.funder-project-idMedical Research Council (MC_UU_12022/6)
cam.issuedOnline2020-09-17


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