Imaging and visualizing SARS-CoV-2 in a new era for structural biology.
dc.contributor.author | Leigh, Kendra E | |
dc.contributor.author | Modis, Yorgo | |
dc.date.accessioned | 2022-01-04T14:32:45Z | |
dc.date.available | 2022-01-04T14:32:45Z | |
dc.date.issued | 2021-12-06 | |
dc.identifier.issn | 2042-8898 | |
dc.identifier.other | rsfs20210019 | |
dc.identifier.uri | https://www.repository.cam.ac.uk/handle/1810/331899 | |
dc.description.abstract | The SARS-CoV-2 pandemic has had a global impact and has put scientific endeavour in the spotlight, perhaps more than any previous viral outbreak. Fortuitously, the pandemic came at a time when decades of research in multiple scientific fields could be rapidly brought to bear, and a new generation of vaccine platforms was on the cusp of clinical maturity. SARS-CoV-2 also emerged at the inflection point of a technological revolution in macromolecular imaging by cryo-electron microscopy, fuelled by a confluence of major technological advances in sample preparation, optics, detectors and image processing software, that complemented pre-existing techniques. Together, these advances enabled us to visualize SARS-CoV-2 and its components more rapidly, in greater detail, and in a wider variety of biologically relevant contexts than would have been possible even a few years earlier. The resulting ultrastructural information on SARS-CoV-2 and how it interacts with the host cell has played a critical role in the much-needed accelerated development of COVID-19 vaccines and therapeutics. Here, we review key imaging modalities used to visualize SARS-CoV-2 and present select example data, which have provided us with an exceptionally detailed picture of this virus. | |
dc.language | en | |
dc.publisher | The Royal Society | |
dc.subject | ARTICLES | |
dc.subject | Review articles | |
dc.subject | SARS-CoV-2 | |
dc.subject | structural biology | |
dc.subject | cryo-electron tomography | |
dc.subject | X-ray crystallography | |
dc.subject | cryo-electron microscopy | |
dc.subject | multiscale imaging | |
dc.title | Imaging and visualizing SARS-CoV-2 in a new era for structural biology. | |
dc.type | Article | |
dc.date.updated | 2022-01-04T14:32:44Z | |
prism.issueIdentifier | 6 | |
prism.publicationName | Interface Focus | |
prism.volume | 11 | |
dc.identifier.doi | 10.17863/CAM.79349 | |
dcterms.dateAccepted | 2021-08-19 | |
rioxxterms.versionofrecord | 10.1098/rsfs.2021.0019 | |
rioxxterms.version | AO | |
rioxxterms.version | VoR | |
rioxxterms.licenseref.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.contributor.orcid | Leigh, Kendra E [0000-0002-6467-3330] | |
dc.contributor.orcid | Modis, Yorgo [0000-0002-6084-0429] | |
dc.identifier.eissn | 2042-8901 | |
pubs.funder-project-id | Wellcome Trust (217191/Z/19/Z) | |
cam.issuedOnline | 2021-10-12 |
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