Genomic analysis finds no evidence of canonical eukaryotic DNA processing complexes in a free-living protist.
Authors
Publication Date
2021-10-14Journal Title
Nat Commun
ISSN
2041-1723
Publisher
Springer Science and Business Media LLC
Volume
12
Issue
1
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Salas-Leiva, D. E., Tromer, E. C., Curtis, B. A., Jerlström-Hultqvist, J., Kolisko, M., Yi, Z., Salas-Leiva, J. S., et al. (2021). Genomic analysis finds no evidence of canonical eukaryotic DNA processing complexes in a free-living protist.. Nat Commun, 12 (1) https://doi.org/10.1038/s41467-021-26077-2
Description
Funder: Canadian Institutes of Health Research (Grant: FRN-142349) Natural Sciences and Engineering Research Council of Canada (Grant: RGPIN 05871-2014)
Abstract
Cells replicate and segregate their DNA with precision. Previous studies showed that these regulated cell-cycle processes were present in the last eukaryotic common ancestor and that their core molecular parts are conserved across eukaryotes. However, some metamonad parasites have secondarily lost components of the DNA processing and segregation apparatuses. To clarify the evolutionary history of these systems in these unusual eukaryotes, we generated a genome assembly for the free-living metamonad Carpediemonas membranifera and carried out a comparative genomics analysis. Here, we show that parasitic and free-living metamonads harbor an incomplete set of proteins for processing and segregating DNA. Unexpectedly, Carpediemonas species are further streamlined, lacking the origin recognition complex, Cdc6 and most structural kinetochore subunits. Carpediemonas species are thus the first known eukaryotes that appear to lack this suite of conserved complexes, suggesting that they likely rely on yet-to-be-discovered or alternative mechanisms to carry out these fundamental processes.
Keywords
Article, /631/181, /631/326, /45/23, /49/91, article
Identifiers
s41467-021-26077-2, 26077
External DOI: https://doi.org/10.1038/s41467-021-26077-2
This record's URL: https://www.repository.cam.ac.uk/handle/1810/332033
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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