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dc.contributor.authorHefele, Lisa
dc.contributor.authorBlack, Antony P
dc.contributor.authorVan Tan, Trinh
dc.contributor.authorTri Minh, Nguyen
dc.contributor.authorDuc Hoang, Nguyen
dc.contributor.authorVirachith, Siriphone
dc.contributor.authorMuller, Claude P
dc.contributor.authorHübschen, Judith M
dc.contributor.authorRussell, Paula
dc.contributor.authorBartholdson Scott, Josefin
dc.contributor.authorNgoc Minh, Chau Nguyen
dc.contributor.authorThieu Nga, Tran Vu
dc.contributor.authorBaker, Stephen
dc.date.accessioned2022-01-05T16:33:47Z
dc.date.available2022-01-05T16:33:47Z
dc.date.issued2021-12
dc.date.submitted2021-03-24
dc.identifier.issn1935-2727
dc.identifier.otherpntd-d-21-00419
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332089
dc.description.abstractThe epidemiology of typhoid fever in Lao People`s Democratic Republic is poorly defined. Estimating the burden of typhoid fever in endemic countries is complex due to the cost and limitations of population-based surveillance; serological approaches may be a more cost-effective alternative. ELISAs were performed on 937 serum samples (317 children and 620 adults) from across Lao PDR to measure IgG antibody titers against Vi polysaccharide and the experimental protein antigens, CdtB and HlyE. We measured the significance of the differences between antibody titers in adults and children and fitted models to assess the relationship between age and antibody titers. The median IgG titres of both anti-HylE and CdtB were significantly higher in children compared to adults (anti-HylE; 351.7 ELISA Units (EU) vs 198.1 EU, respectively; p<0.0001 and anti-CdtB; 52.6 vs 12.9 EU; p<0.0001). Conversely, the median anti-Vi IgG titer was significantly higher in adults than children (11.3 vs 3.0 U/ml; p<0.0001). A non-linear trend line fitted to the anti-CdtB and anti-HlyE IgG data identified a peak in antibody concentration in children <5 years of age. We identified elevated titers of anti-HlyE and anti-CdtB IgG in the serum of children residing in Lao PDR in comparison to adults. These antigens are associated with seroconversion after typhoid fever and may be a superior measure of disease burden than anti-Vi IgG. This approach is scalable and may be developed to assess the burden of typhoid fever in countries where the disease may be endemic, and evidence is required for the introduction of typhoid vaccines.
dc.languageen
dc.publisherPublic Library of Science (PLoS)
dc.subjectResearch Article
dc.subjectMedicine and health sciences
dc.subjectResearch and analysis methods
dc.subjectBiology and life sciences
dc.subjectPeople and places
dc.titleAn age-stratified serosurvey against purified Salmonella enterica serovar Typhi antigens in the Lao People´s Democratic Republic.
dc.typeArticle
dc.date.updated2022-01-05T16:33:46Z
prism.issueIdentifier12
prism.publicationNamePLoS Negl Trop Dis
prism.volume15
dc.identifier.doi10.17863/CAM.79536
dcterms.dateAccepted2021-11-22
rioxxterms.versionofrecord10.1371/journal.pntd.0010017
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
datacite.contributor.supervisoreditor: Bourret, Travis J.
dc.contributor.orcidTri Minh, Nguyen [0000-0002-1819-2296]
dc.contributor.orcidDuc Hoang, Nguyen [0000-0003-2437-3568]
dc.contributor.orcidVirachith, Siriphone [0000-0001-5049-0544]
dc.contributor.orcidHübschen, Judith M [0000-0002-5001-2128]
dc.contributor.orcidRussell, Paula [0000-0003-2389-6631]
dc.contributor.orcidBartholdson Scott, Josefin [0000-0003-3380-4446]
dc.contributor.orcidThieu Nga, Tran Vu [0000-0003-3672-9867]
dc.contributor.orcidBaker, Stephen [0000-0003-1308-5755]
dc.identifier.eissn1935-2735
pubs.funder-project-idMinistry of Foreign and European Affairs (Luxembourg-Laos Partnership for Research and Capacity Building in Infectious Disease Surveillance II)
pubs.funder-project-idWellcome Trust (215515/Z/19/Z)
cam.issuedOnline2021-12-13


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