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dc.contributor.authorVanderpoorten, Oliver
dc.contributor.authorBabar, Ali N
dc.contributor.authorKrainer, Georg
dc.contributor.authorJacquat, Rapha<U+FFFD>l
dc.contributor.authorChalla, Pavan K
dc.contributor.authorPeter, Quentin
dc.contributor.authorToprakcioglu, Zenon
dc.contributor.authorXu, Catherine K
dc.contributor.authorKeyser, Ulrich
dc.contributor.authorBaumberg, Jeremy
dc.contributor.authorKaminski, Clemens
dc.contributor.authorKnowles, Tuomas
dc.date.accessioned2022-01-06T00:30:11Z
dc.date.available2022-01-06T00:30:11Z
dc.date.issued2022-02-25
dc.identifier.issn2574-0970
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332106
dc.description.abstractThe analysis of nanoscopic species, such as proteins and colloidal assemblies, at the single-molecule level has become vital in many areas of fundamental and applied research. Approaches to increase the detection timescales for single molecules in solution without immobilising them onto a substrate surface and applying external fields are much sought after. Here we present an easy-to-implement and versatile nanofluidics-based approach that enables increased observational-timescale analysis of single biomacromolecules and nanoscale colloids in solution. We use two-photon-based hybrid lithography in conjunction with soft lithography to fabricate nanofluidic devices with nano-trapping geometries down to 100 nm in height. We provide a rigorous description and characterisation of the fabrication route that enables the writing of nanoscopic 3D structures directly in photoresist and allows for the integration of nano-trapping and nano-channel geometries within micro-channel devices. Using confocal fluorescence burst detection, we validated the functionality of particle confinement in our nano-trap geometries through measurement of particle residence times. All species under study, including nanoscale colloids, α-synuclein oligomers, and double-stranded DNA, showed a three to five-fold increase in average residence time in the detection volume of nano-traps, due to the additional local steric confinement, in comparison to free space diffusion in a nearby micro-channel. Our approach thus opens-up the possibility for single-molecule studies at prolonged observational timescales to analyse and detect nanoparticles and protein assemblies in solution without the need for surface immobilisation.
dc.description.sponsorshipHorizon 2020 programme (766972-FET-OPEN-NANOPHLOW); EPSRC EP/L015889/1; Horizon 2020 Framework Programme through the Marie Sklodowska-Curie grant MicroSPARK (agreement n◦ 841466;, the Herchel Smith Funds, and the Wolfson College Junior Research Fellowship
dc.publisherACS
dc.rightsAll Rights Reserved
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserved
dc.titleNanofluidic Traps by Two-Photon Fabrication for Extended Detection of Macromolecules and Colloids in Solution
dc.typeArticle
dc.publisher.departmentDepartment of Chemistry
dc.publisher.departmentDepartment of Chemistry Student
dc.publisher.departmentDepartment of Physics
dc.publisher.departmentDepartment of Chemical Engineering And Biotechnology
dc.date.updated2021-12-09T13:26:31Z
prism.publicationNameACS APPLIED NANO MATERIALS
dc.identifier.doi10.17863/CAM.79552
dcterms.dateAccepted2021-11-24
rioxxterms.versionofrecord10.1021/acsanm.1c03691
rioxxterms.versionAM
dc.contributor.orcidKrainer, Georg [0000-0002-9626-7636]
dc.contributor.orcidJacquat, Rapha<U+FFFD>l [0000-0002-8661-9722]
dc.contributor.orcidPeter, Quentin [0000-0002-8018-3059]
dc.contributor.orcidToprakcioglu, Zenon [0000-0003-1964-8432]
dc.contributor.orcidKeyser, Ulrich [0000-0003-3188-5414]
dc.contributor.orcidBaumberg, Jeremy [0000-0002-9606-9488]
dc.contributor.orcidKaminski, Clemens [0000-0002-5194-0962]
dc.contributor.orcidKnowles, Tuomas [0000-0002-7879-0140]
dc.identifier.eissn2574-0970
rioxxterms.typeJournal Article/Review
cam.issuedOnline2022-02-03
cam.orpheus.success2022-02-03: embargo amended to 12 months
cam.orpheus.counter2
cam.depositDate2021-12-09
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement
rioxxterms.freetoread.startdate2023-02-03


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