Symmetry breaking in the female germline cyst.
View / Open Files
Publication Date
2021-11-12Journal Title
Science
ISSN
0036-8075
Publisher
American Association for the Advancement of Science (AAAS)
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Nashchekin, D., Busby, L., Jakobs, M., Squires, I., & St Johnston, D. (2021). Symmetry breaking in the female germline cyst.. Science https://doi.org/10.1126/science.abj3125
Abstract
In mammals and flies, only one cell in a multicellular female germline cyst becomes an oocyte, but how symmetry is broken to select the oocyte is unknown. Here, we show that the microtubule (MT) minus end-stabilizing protein Patronin/CAMSAP marks the future Drosophila oocyte and is required for oocyte specification. The spectraplakin Shot recruits Patronin to the fusome, a branched structure extending into all cyst cells. Patronin stabilizes more MTs in the cell with the most fusome material. Our data suggest that this weak asymmetry is amplified by Dynein-dependent transport of Patronin-stabilized MTs. This forms a polarized MT network, along which Dynein transports oocyte determinants into the presumptive oocyte. Thus, Patronin amplifies a weak fusome anisotropy to break symmetry and select one cell to become the oocyte.
Keywords
Animals, Anisotropy, Drosophila Proteins, Drosophila melanogaster, Dyneins, Female, Germ Cells, Microfilament Proteins, Microtubule-Associated Proteins, Microtubule-Organizing Center, Microtubules, Oocytes, Organelles
Sponsorship
Wellcome Trust (092096/Z/10/Z)
Wellcome Trust (080007/B/06/Z)
Wellcome Trust (203144/Z/16/Z)
Wellcome Trust (207496/Z/17/Z)
Biotechnology and Biological Sciences Research Council (BB/R001618/1)
Cancer Research Uk (None)
Cancer Research UK (C6946/A24843)
Wellcome Trust (109145/Z/15/Z)
Identifiers
PMC7612008, 34762476
External DOI: https://doi.org/10.1126/science.abj3125
This record's URL: https://www.repository.cam.ac.uk/handle/1810/332173
Statistics
Total file downloads (since January 2020). For more information on metrics see the
IRUS guide.
Recommended or similar items
The current recommendation prototype on the Apollo Repository will be turned off on 03 February 2023. Although the pilot has been fruitful for both parties, the service provider IKVA is focusing on horizon scanning products and so the recommender service can no longer be supported. We recognise the importance of recommender services in supporting research discovery and are evaluating offerings from other service providers. If you would like to offer feedback on this decision please contact us on: support@repository.cam.ac.uk