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dc.contributor.authorPogodalla, Nicole
dc.contributor.authorKranenburg, Holger
dc.contributor.authorRey, Simone
dc.contributor.authorRodrigues, Silke
dc.contributor.authorCardona, Albert
dc.contributor.authorKlämbt, Christian
dc.date.accessioned2022-01-06T12:55:10Z
dc.date.available2022-01-06T12:55:10Z
dc.date.issued2021-11-04
dc.identifier.issn2041-1723
dc.identifier.otherPMC8569210
dc.identifier.other34737284
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332203
dc.description.abstractIn the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute to the formation of an internal diffusion barrier. We find that ensheathing glia are polarized with a basolateral plasma membrane rich in phosphatidylinositol-(3,4,5)-triphosphate (PIP<sub>3</sub>) and the Na<sup>+</sup>/K<sup>+</sup>-ATPase Nervana2 (Nrv2) that abuts an extracellular matrix formed at neuropil-cortex interface. The apical plasma membrane is facing the neuropil and is rich in phosphatidylinositol-(4,5)-bisphosphate (PIP<sub>2</sub>) that is supported by a sub-membranous ß<sub>Heavy</sub>-Spectrin cytoskeleton. ß<sub>Heavy</sub>-spectrin mutant larvae affect ensheathing glial cell polarity with delocalized PIP<sub>2</sub> and Nrv2 and exhibit an abnormal locomotion which is similarly shown by ensheathing glia ablated larvae. Thus, polarized glia compartmentalizes the brain and is essential for proper nervous system function.
dc.languageeng
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2041-1723
dc.sourcenlmid: 101528555
dc.subjectBrain
dc.subjectNeuroglia
dc.subjectNeuropil
dc.subjectNeurons
dc.subjectAnimals
dc.subjectDrosophila
dc.subjectGlycoproteins
dc.subjectPhosphatidylinositol Phosphates
dc.subjectSpectrin
dc.subjectDrosophila Proteins
dc.subjectNerve Tissue Proteins
dc.subjectCell Lineage
dc.subjectLarva
dc.titleDrosophila ß<sub>Heavy</sub>-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil.
dc.typeArticle
dc.date.updated2022-01-06T12:55:09Z
prism.issueIdentifier1
prism.publicationNameNature communications
prism.volume12
dc.identifier.doi10.17863/CAM.79649
rioxxterms.versionofrecord10.1038/s41467-021-26462-x
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidCardona, Albert [0000-0003-4941-6536]
dc.contributor.orcidKlämbt, Christian [0000-0002-6349-5800]


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International