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dc.contributor.authorGeorge, Ginto
dc.contributor.authorNinagawa, Satoshi
dc.contributor.authorYagi, Hirokazu
dc.contributor.authorFurukawa, Jun-Ichi
dc.contributor.authorHashii, Noritaka
dc.contributor.authorIshii-Watabe, Akiko
dc.contributor.authorDeng, Ying
dc.contributor.authorMatsushita, Kazutoshi
dc.contributor.authorIshikawa, Tokiro
dc.contributor.authorMamahit, Yugoviandi P
dc.contributor.authorMaki, Yuta
dc.contributor.authorKajihara, Yasuhiro
dc.contributor.authorKato, Koichi
dc.contributor.authorOkada, Tetsuya
dc.contributor.authorMori, Kazutoshi
dc.date.accessioned2022-01-07T00:31:26Z
dc.date.available2022-01-07T00:31:26Z
dc.date.issued2021-10-26
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332258
dc.description.abstractSequential mannose trimming of N-glycan, from M9 to M8B and then to oligosaccharides exposing the α1,6-linked mannosyl residue (M7A, M6, and M5), facilitates endoplasmic reticulum-associated degradation of misfolded glycoproteins (gpERAD). We previously showed that EDEM2 stably disulfide-bonded to the thioredoxin domain-containing protein TXNDC11 is responsible for the first step (George et al., 2020). Here, we show that EDEM3 and EDEM1 are responsible for the second step. Incubation of pyridylamine-labeled M8B with purified EDEM3 alone produced M7 (M7A and M7C), M6, and M5. EDEM1 showed a similar tendency, although much lower amounts of M6 and M5 were produced. Thus, EDEM3 is a major α1,2-mannosidase for the second step from M8B. Both EDEM3 and EDEM1 trimmed M8B from a glycoprotein efficiently. Our confirmation of the Golgi localization of MAN1B indicates that no other α1,2-mannosidase is required for gpERAD. Accordingly, we have established the entire route of oligosaccharide processing and the enzymes responsible.
dc.format.mediumElectronic
dc.publishereLife Sciences Publications, Ltd
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectbiochemistry
dc.subjectcell biology
dc.subjectchemical biology
dc.subjectglycoprotein
dc.subjecthuman
dc.subjectmannose trimming
dc.subjectprotein degradation
dc.subjectCalcium-Binding Proteins
dc.subjectCell Line
dc.subjectEndoplasmic Reticulum-Associated Degradation
dc.subjectGlycoproteins
dc.subjectHumans
dc.subjectMembrane Proteins
dc.subjectOligosaccharides
dc.subjectalpha-Mannosidase
dc.titlePurified EDEM3 or EDEM1 alone produces determinant oligosaccharide structures from M8B in mammalian glycoprotein ERAD.
dc.typeArticle
dc.publisher.departmentC.I.M.R. Clinical Biochemistry
dc.date.updated2022-01-06T11:10:32Z
prism.numberARTN e70357
prism.publicationDate2021
prism.publicationNameElife
prism.startingPagee70357
prism.volume10
dc.identifier.doi10.17863/CAM.79703
dcterms.dateAccepted2021-10-07
rioxxterms.versionofrecord10.7554/eLife.70357
rioxxterms.versionVoR
dc.contributor.orcidGeorge, Ginto [0000-0003-4804-9594]
dc.contributor.orcidNinagawa, Satoshi [0000-0002-8005-4716]
dc.contributor.orcidYagi, Hirokazu [0000-0001-9296-0225]
dc.contributor.orcidFurukawa, Jun-Ichi [0000-0002-7284-2261]
dc.contributor.orcidHashii, Noritaka [0000-0003-1363-2050]
dc.contributor.orcidDeng, Ying [0000-0003-4657-9284]
dc.contributor.orcidMatsushita, Kazutoshi [0000-0002-5666-9630]
dc.contributor.orcidIshikawa, Tokiro [0000-0003-1718-6764]
dc.contributor.orcidMamahit, Yugoviandi P [0000-0002-2974-7795]
dc.contributor.orcidMaki, Yuta [0000-0002-5838-302X]
dc.contributor.orcidKajihara, Yasuhiro [0000-0002-6656-2394]
dc.contributor.orcidKato, Koichi [0000-0001-7187-9612]
dc.contributor.orcidOkada, Tetsuya [0000-0002-2513-1301]
dc.contributor.orcidMori, Kazutoshi [0000-0001-7378-4019]
dc.identifier.eissn2050-084X
rioxxterms.typeJournal Article/Review
cam.issuedOnline2021-10-26
cam.depositDate2022-01-06
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International