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dc.contributor.authorTuong, Kelvin
dc.contributor.authorLukowski, Samuel W
dc.contributor.authorNguyen, Quan H
dc.contributor.authorChandra, Janin
dc.contributor.authorZhou, Chenhao
dc.contributor.authorGillinder, Kevin
dc.contributor.authorBashaw, Abate A
dc.contributor.authorFerdinand, John
dc.contributor.authorStewart, Benjamin J
dc.contributor.authorTeoh, Siok Min
dc.contributor.authorHanson, Sarah J
dc.contributor.authorDevitt, Katharina
dc.contributor.authorClatworthy, Menna R
dc.contributor.authorPowell, Joseph E
dc.contributor.authorFrazer, Ian H
dc.date.accessioned2022-01-07T00:31:52Z
dc.date.available2022-01-07T00:31:52Z
dc.date.issued2021-11-19
dc.identifier.issn2589-0042
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332263
dc.description.abstractLangerhans cells (LC) are skin-resident antigen-presenting cells that regulate immune responses to epithelial microorganisms. Human papillomavirus (HPV) infection can promote malignant epithelial transformation. As LCs are considered important for controlling HPV infection, we compared the transcriptome of murine LCs from skin transformed by K14E7 oncoprotein and from healthy skin. We identified transcriptome heterogeneity at the single cell level amongst LCs in normal skin, associated with ontogeny, cell cycle, and maturation. We identified a balanced co-existence of immune-stimulatory and immune-inhibitory LC cell states in normal skin that was significantly disturbed in HPV16 E7-transformed skin. Hyperplastic skin was depleted of immune-stimulatory LCs and enriched for LCs with an immune-inhibitory gene signature, and LC-keratinocyte crosstalk was dysregulated. We identified reduced expression of interleukin (IL)-34, a critical molecule for LC homeostasis. Enrichment of an immune-inhibitory LC gene signature and reduced levels of epithelial IL-34 were also found in human HPV-associated cervical epithelial cancers.
dc.format.mediumElectronic-eCollection
dc.publisherElsevier BV
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBioinformatics
dc.subjectBiological sciences
dc.subjectImmune system
dc.subjectTranscriptomics
dc.titleA model of impaired Langerhans cell maturation associated with HPV induced epithelial hyperplasia.
dc.typeArticle
dc.publisher.departmentDepartment of Medicine
dc.date.updated2022-01-06T11:41:53Z
prism.issueIdentifier11
prism.numberARTN 103326
prism.publicationDate2021
prism.publicationNameiScience
prism.startingPage103326
prism.volume24
dc.identifier.doi10.17863/CAM.79708
dcterms.dateAccepted2021-10-19
rioxxterms.versionofrecord10.1016/j.isci.2021.103326
rioxxterms.versionVoR
dc.contributor.orcidTuong, Kelvin [0000-0002-6735-6808]
dc.contributor.orcidFerdinand, John [0000-0003-0936-0128]
dc.identifier.eissn2589-0042
rioxxterms.typeJournal Article/Review
cam.issuedOnline2021-10-21
cam.depositDate2022-01-06
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International