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dc.contributor.authorTivony, Ran
dc.contributor.authorFletcher, Marcus
dc.contributor.authorAl Nahas, Kareem
dc.contributor.authorKeyser, Ulrich F
dc.date.accessioned2022-01-07T16:46:24Z
dc.date.available2022-01-07T16:46:24Z
dc.date.issued2021-11-19
dc.identifier.issn2161-5063
dc.identifier.otherPMC8609574
dc.identifier.other34761904
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332321
dc.descriptionFunder: University of Cambridge
dc.descriptionFunder: Blavatnik Family Foundation
dc.description.abstractCell-sized vesicles like giant unilamellar vesicles (GUVs) are established as a promising biomimetic model for studying cellular phenomena in isolation. However, the presence of residual components and byproducts, generated during vesicles preparation and manipulation, severely limits the utility of GUVs in applications like synthetic cells. Therefore, with the rapidly growing field of synthetic biology, there is an emergent demand for techniques that can continuously purify cell-like vesicles from diverse residues, while GUVs are being simultaneously synthesized and manipulated. We have developed a microfluidic platform capable of purifying GUVs through stream bifurcation, where a vesicles suspension is partitioned into three fractions: purified GUVs, residual components, and a washing solution. Using our purification approach, we show that giant vesicles can be separated from various residues─which range in size and chemical composition─with a very high efficiency (e = 0.99), based on size and deformability of the filtered objects. In addition, by incorporating the purification module with a microfluidic-based GUV-formation method, octanol-assisted liposome assembly (OLA), we established an integrated production-purification microfluidic unit that sequentially produces, manipulates, and purifies GUVs. We demonstrate the applicability of the integrated device to synthetic biology through sequentially fusing SUVs with freshly prepared GUVs and separating the fused GUVs from extraneous SUVs and oil droplets at the same time.
dc.languageeng
dc.publisherAmerican Chemical Society (ACS)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2161-5063
dc.sourcenlmid: 101575075
dc.subjectMicrofluidics
dc.subjectGiant Unilamellar Vesicles
dc.subjectLipid Bilayer
dc.subjectBottom-up Synthesis
dc.subjectArtificial Cell Models
dc.subjectGiant Vesicle Purification
dc.titleA Microfluidic Platform for Sequential Assembly and Separation of Synthetic Cell Models.
dc.typeArticle
dc.date.updated2022-01-07T16:46:23Z
prism.endingPage3116
prism.issueIdentifier11
prism.publicationNameACS Synth Biol
prism.startingPage3105
prism.volume10
dc.identifier.doi10.17863/CAM.79767
rioxxterms.versionofrecord10.1021/acssynbio.1c00371
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidTivony, Ran [0000-0003-0331-9538]
dc.contributor.orcidFletcher, Marcus [0000-0003-3036-1168]
dc.contributor.orcidAl Nahas, Kareem [0000-0003-4568-5894]
dc.contributor.orcidKeyser, Ulrich F [0000-0003-3188-5414]
dc.identifier.eissn2161-5063
pubs.funder-project-idEuropean Research Council (647144)
pubs.funder-project-idEuropean Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (892333)
cam.issuedOnline2021-11-11


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International