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dc.contributor.authorTruong Quang, Binh An
dc.contributor.authorPeters, Ruby
dc.contributor.authorCassani, Davide AD
dc.contributor.authorChugh, Priyamvada
dc.contributor.authorClark, Andrew G
dc.contributor.authorAgnew, Meghan
dc.contributor.authorCharras, Guillaume
dc.contributor.authorPaluch, Ewa Kamila
dc.date.accessioned2022-01-07T16:46:43Z
dc.date.available2022-01-07T16:46:43Z
dc.date.issued2021-11-11
dc.identifier.issn2041-1723
dc.identifier.otherPMC8586027
dc.identifier.other34764258
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332325
dc.description.abstractIn animal cells, shape is mostly determined by the actomyosin cortex, a thin cytoskeletal network underlying the plasma membrane. Myosin motors generate tension in the cortex, and tension gradients result in cellular deformations. As such, many cell morphogenesis studies have focused on the mechanisms controlling myosin activity and recruitment to the cortex. Here, we demonstrate using super-resolution microscopy that myosin does not always overlap with actin at the cortex, but remains restricted towards the cytoplasm in cells with low cortex tension. We propose that this restricted penetration results from steric hindrance, as myosin minifilaments are considerably larger than the cortical actin meshsize. We identify myosin activity and actin network architecture as key regulators of myosin penetration into the cortex, and show that increasing myosin penetration increases cortical tension. Our study reveals that the spatial coordination of myosin and actin at the cortex regulates cell surface mechanics, and unveils an important mechanism whereby myosin size controls its action by limiting minifilament penetration into the cortical actin network. More generally, our findings suggest that protein size could regulate function in dense cytoskeletal structures.
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2041-1723
dc.sourcenlmid: 101528555
dc.subjectCell Membrane
dc.subjectAnimals
dc.subjectActins
dc.subjectMyosins
dc.subjectActin Cytoskeleton
dc.titleExtent of myosin penetration within the actin cortex regulates cell surface mechanics.
dc.typeArticle
dc.date.updated2022-01-07T16:46:42Z
prism.issueIdentifier1
prism.publicationNameNat Commun
prism.volume12
dc.identifier.doi10.17863/CAM.79771
dcterms.dateAccepted2021-10-04
rioxxterms.versionofrecord10.1038/s41467-021-26611-2
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidClark, Andrew G [0000-0003-3712-4931]
dc.contributor.orcidCharras, Guillaume [0000-0002-7902-0279]
dc.contributor.orcidPaluch, Ewa Kamila [0000-0003-4691-2323]
dc.identifier.eissn2041-1723
pubs.funder-project-idEuropean Research Council (820188)
cam.issuedOnline2021-11-11


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International