Show simple item record

dc.contributor.authorWade, A Ayanna
dc.contributor.authorVan Den Ameele, Jelle
dc.contributor.authorCheetham, Seth W
dc.contributor.authorYakob, Rebecca
dc.contributor.authorBrand, Andrea
dc.contributor.authorNord, Alex S
dc.date.accessioned2022-01-07T16:47:15Z
dc.date.available2022-01-07T16:47:15Z
dc.date.issued2021-11-19
dc.identifier.issn2589-0042
dc.identifier.otherPMC8551073
dc.identifier.other34746699
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332332
dc.descriptionFunder: Royal Society
dc.descriptionFunder: Agouron Institute
dc.description.abstractGenetic studies of autism have revealed causal roles for chromatin remodeling gene mutations. Chromodomain helicase DNA binding protein 8 (CHD8) encodes a chromatin remodeler with significant de novo mutation rates in sporadic autism. However, relationships between CHD8 genomic function and autism-relevant biology remain poorly elucidated. Published studies utilizing ChIP-seq to map CHD8 protein-DNA interactions have high variability, consistent with technical challenges and limitations associated with this method. Thus, complementary approaches are needed to establish CHD8 genomic targets and regulatory functions in developing brain. We used in utero CHD8 Targeted DamID followed by sequencing (TaDa-seq) to characterize CHD8 binding in embryonic mouse cortex. CHD8 TaDa-seq reproduced interaction patterns observed from ChIP-seq and further highlighted CHD8 distal interactions associated with neuronal loci. This study establishes TaDa-seq as a useful alternative for mapping protein-DNA interactions in vivo and provides insights into the regulatory targets of CHD8 and autism-relevant pathophysiology associated with CHD8 mutations.
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceessn: 2589-0042
dc.sourcenlmid: 101724038
dc.subjectBiotechnology
dc.subjectGenomic analysis
dc.subjectGenomics
dc.subjectMolecular Neuroscience
dc.titleIn vivo targeted DamID identifies CHD8 genomic targets in fetal mouse brain.
dc.typeArticle
dc.date.updated2022-01-07T16:47:15Z
prism.issueIdentifier11
prism.publicationNameiScience
prism.volume24
dc.identifier.doi10.17863/CAM.79778
dcterms.dateAccepted2021-10-04
rioxxterms.versionofrecord10.1016/j.isci.2021.103234
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.contributor.orcidVan Den Ameele, Jelle [0000-0002-2744-0810]
dc.contributor.orcidBrand, Andrea [0000-0002-2089-6954]
dc.identifier.eissn2589-0042
pubs.funder-project-idWellcome Trust (103792/Z/14/Z)
pubs.funder-project-idWellcome Trust (105839/Z/14/Z)
pubs.funder-project-idRoyal Society (RP150061)
pubs.funder-project-idWellcome Trust (092096/Z/10/Z)
pubs.funder-project-idCancer Research Uk (None)


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International