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dc.contributor.authorBrohl, Andrew S
dc.contributor.authorSindiri, Sivasish
dc.contributor.authorWei, Jun S
dc.contributor.authorMilewski, David
dc.contributor.authorChou, Hsien-Chao
dc.contributor.authorSong, Young K
dc.contributor.authorWen, Xinyu
dc.contributor.authorKumar, Jeetendra
dc.contributor.authorReardon, Hue V
dc.contributor.authorMudunuri, Uma S
dc.contributor.authorCollins, Jack R
dc.contributor.authorNagaraj, Sushma
dc.contributor.authorGangalapudi, Vineela
dc.contributor.authorTyagi, Manoj
dc.contributor.authorZhu, Yuelin J
dc.contributor.authorMasih, Katherine
dc.contributor.authorYohe, Marielle E
dc.contributor.authorShern, Jack F
dc.contributor.authorQi, Yue
dc.contributor.authorGuha, Udayan
dc.contributor.authorCatchpoole, Daniel
dc.contributor.authorOrentas, Rimas J
dc.contributor.authorKuznetsov, Igor B
dc.contributor.authorLlosa, Nicolas J
dc.contributor.authorLigon, John A
dc.contributor.authorTurpin, Brian K
dc.contributor.authorLeino, Daniel G
dc.contributor.authorIwata, Shintaro
dc.contributor.authorAndrulis, Irene L
dc.contributor.authorWunder, Jay S
dc.contributor.authorToledo, Silvia RC
dc.contributor.authorMeltzer, Paul S
dc.contributor.authorLau, Ching
dc.contributor.authorTeicher, Beverly A
dc.contributor.authorMagnan, Heather
dc.contributor.authorLadanyi, Marc
dc.contributor.authorKhan, Javed
dc.date.accessioned2022-01-07T17:55:59Z
dc.date.available2022-01-07T17:55:59Z
dc.date.issued2021-11-23
dc.identifier.issn2211-1247
dc.identifier.otherPMC8642810
dc.identifier.other34818552
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332441
dc.description.abstractWe perform an immunogenomics analysis utilizing whole-transcriptome sequencing of 657 pediatric extracranial solid cancer samples representing 14 diagnoses, and additionally utilize transcriptomes of 131 pediatric cancer cell lines and 147 normal tissue samples for comparison. We describe patterns of infiltrating immune cells, T cell receptor (TCR) clonal expansion, and translationally relevant immune checkpoints. We find that tumor-infiltrating lymphocytes and TCR counts vary widely across cancer types and within each diagnosis, and notably are significantly predictive of survival in osteosarcoma patients. We identify potential cancer-specific immunotherapeutic targets for adoptive cell therapies including cell-surface proteins, tumor germline antigens, and lineage-specific transcription factors. Using an orthogonal immunopeptidomics approach, we find several potential immunotherapeutic targets in osteosarcoma and Ewing sarcoma and validated PRAME as a bona fide multi-pediatric cancer target. Importantly, this work provides a critical framework for immune targeting of extracranial solid tumors using parallel immuno-transcriptomic and -peptidomic approaches.
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2211-1247
dc.sourcenlmid: 101573691
dc.subjectT cell receptor
dc.subjectRna Sequencing
dc.subjectAdoptive Cell Therapy
dc.subjectPediatric Oncology
dc.subjectTumor-infiltrating Lymphocytes
dc.subjectPrame
dc.subjectImmunogenomics
dc.subjectImmunopeptidomics
dc.titleImmuno-transcriptomic profiling of extracranial pediatric solid malignancies.
dc.typeArticle
dc.date.updated2022-01-07T17:55:59Z
prism.issueIdentifier8
prism.publicationNameCell Rep
prism.volume37
dc.identifier.doi10.17863/CAM.79887
dcterms.dateAccepted2021-11-01
rioxxterms.versionofrecord10.1016/j.celrep.2021.110047
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidMasih, Katherine [0000-0002-0809-668X]
dc.identifier.eissn2211-1247
pubs.funder-project-idIntramural NIH HHS (ZIA BC010806, ZIA BC010593, ZIA BC011002, Z01 SC010366, Z99 CA999999, ZIA SC010366, Z01 BC010593, Z01 BC011002, ZIA BC010998)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International