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dc.contributor.authorCan, Emine
dc.contributor.authorBastiaansen, Jessica AM
dc.contributor.authorCouturier, Dominique
dc.contributor.authorGruetter, Rolf
dc.contributor.authorYoshihara, Hikari AI
dc.contributor.authorComment, Arnaud
dc.date.accessioned2022-01-10T17:44:22Z
dc.date.available2022-01-10T17:44:22Z
dc.date.issued2022-01-10
dc.date.submitted2021-07-31
dc.identifier.issn2399-3642
dc.identifier.others42003-021-02978-2
dc.identifier.other2978
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332579
dc.descriptionFunder: EC | EC Seventh Framework Programm | FP7 People: Marie-Curie Actions (FP7-PEOPLE - Specific Programme "People" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013)); doi: https://doi.org/10.13039/100011264; Grant(s): 264780
dc.description.abstractHyperpolarized [1-13C]pyruvate enables direct in vivo assessment of real-time liver enzymatic activities by 13C magnetic resonance. However, the technique usually requires the injection of a highly supraphysiological dose of pyruvate. We herein demonstrate that liver metabolism can be measured in vivo with hyperpolarized [1-13C]pyruvate administered at two- to three-fold the basal plasma concentration. The flux through pyruvate dehydrogenase, assessed by 13C-labeling of bicarbonate in the fed condition, was found to be saturated or partially inhibited by supraphysiological doses of hyperpolarized [1-13C]pyruvate. The [13C]bicarbonate signal detected in the liver of fasted rats nearly vanished after treatment with a phosphoenolpyruvate carboxykinase (PEPCK) inhibitor, indicating that the signal originates from the flux through PEPCK. In addition, the normalized [13C]bicarbonate signal in fasted untreated animals is dose independent across a 10-fold range, highlighting that PEPCK and pyruvate carboxylase are not saturated and that hepatic gluconeogenesis can be directly probed in vivo with hyperpolarized [1-13C]pyruvate.
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.subjectArticle
dc.subject/692/4020/4021/288/2032
dc.subject/692/308/2778
dc.subject/631/45/320
dc.subject/631/1647/245/1628
dc.subject/59/57
dc.subject/82/58
dc.subject/82/6
dc.subjectarticle
dc.title[13C]bicarbonate labelled from hyperpolarized [1-13C]pyruvate is an in vivo marker of hepatic gluconeogenesis in fasted state.
dc.typeArticle
dc.date.updated2022-01-10T17:43:20Z
prism.issueIdentifier1
prism.publicationNameCommun Biol
prism.volume5
dc.identifier.doi10.17863/CAM.80029
dcterms.dateAccepted2021-12-07
rioxxterms.versionofrecord10.1038/s42003-021-02978-2
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidCan, Emine [0000-0002-3519-8294]
dc.contributor.orcidCouturier, Dominique [0000-0001-5774-5036]
dc.contributor.orcidYoshihara, Hikari AI [0000-0002-3274-7147]
dc.contributor.orcidComment, Arnaud [0000-0002-8484-3448]
dc.identifier.eissn2399-3642
pubs.funder-project-idEuropean Commission (264780)
pubs.funder-project-idEuropean Research Council (682574)
pubs.funder-project-idSwiss National Science Foundation (133562)
cam.issuedOnline2022-01-10


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