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dc.contributor.authorClark, John
dc.contributor.authorKean, Iain Robert Louis
dc.contributor.authorCurran, Martin D
dc.contributor.authorKhokhar, Fahad
dc.contributor.authorWhite, Deborah
dc.contributor.authorDaubney, Esther
dc.contributor.authorConway Morris, Andrew
dc.contributor.authorNavapurkar, Vilas
dc.contributor.authorBartholdson Scott, Josefin
dc.contributor.authorMaes, Mailis
dc.contributor.authorBousfield, Rachel
dc.contributor.authorGouliouris, Theodore
dc.contributor.authorAgrawal, Shruti
dc.contributor.authorInwald, David
dc.contributor.authorZhang, Zhenguang
dc.contributor.authorTörök, M Estée
dc.contributor.authorBaker, Stephen
dc.contributor.authorPathan, Nazima
dc.date.accessioned2022-01-11T00:31:50Z
dc.date.available2022-01-11T00:31:50Z
dc.date.issued2021-11-29
dc.identifier.issn2044-6055
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332599
dc.description.abstractINTRODUCTION: Lower respiratory tract infection (LRTI) is the most commonly treated infection in critically ill children. Pathogens are infrequently identified on routine respiratory culture, and this is a time-consuming process. A syndromic approach to rapid molecular testing that includes a wide range of bacterial and fungal targets has the potential to aid clinical decision making and reduce unnecessary broad spectrum antimicrobial prescribing. Here, we describe a single-centre prospective cohort study investigating the use of a 52-pathogen TaqMan array card (TAC) for LRTI in the paediatric intensive care unit (PICU). METHODS AND ANALYSIS: Critically ill children with suspected LRTI will be enrolled to this 100 patient single-centre prospective observational study in a PICU in the East of England. Samples will be obtained via routine non-bronchoscopic bronchoalveolar lavage which will be sent for standard microbiology culture in addition to TAC. A blood draw will be obtained via any existing vascular access device. The primary outcomes of the study will be (1) concordance of TAC result with routine culture and 16S rRNA gene sequencing and (2) time of diagnostic result from TAC versus routine culture. Secondary outcomes will include impact of the test on total antimicrobial prescriptions, a description of the inflammatory profile of the lung and blood in response to pneumonia and a description of the clinical experience of medical and nursing staff using TAC. ETHICS AND DISSEMINATION: This study has been approved by the Yorkshire and the Humber-Bradford Leeds Research Ethics Committee (REC reference 20/YH/0089). Informed consent will be obtained from all participants. Results will be published in peer-reviewed publications and international conferences. TRIAL REGISTRATION NUMBER: NCT04233268.
dc.format.mediumElectronic
dc.publisherBMJ
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectmolecular diagnostics
dc.subjectpaediatric intensive & critical care
dc.subjectrespiratory infections
dc.subjectChild
dc.subjectCohort Studies
dc.subjectHumans
dc.subjectLung
dc.subjectObservational Studies as Topic
dc.subjectPneumonia
dc.subjectProspective Studies
dc.subjectRNA, Ribosomal, 16S
dc.titleRapid Assay for Sick Children with Acute Lung infection Study (RASCALS): diagnostic cohort study protocol.
dc.typeArticle
dc.publisher.departmentDepartment of Paediatrics Student
dc.publisher.departmentDepartment of Medicine
dc.publisher.departmentDepartment of Paediatrics
dc.date.updated2022-01-08T14:07:24Z
prism.issueIdentifier11
prism.numberARTN e056197
prism.publicationDate2021
prism.publicationNameBMJ Open
prism.startingPagee056197
prism.volume11
dc.identifier.doi10.17863/CAM.80046
rioxxterms.versionofrecord10.1136/bmjopen-2021-056197
rioxxterms.versionVoR
dc.contributor.orcidClark, John [0000-0001-6916-9195]
dc.contributor.orcidConway Morris, Andrew [0000-0002-3211-3216]
dc.contributor.orcidTörök, M Estée [0000-0001-9098-8590]
dc.contributor.orcidBaker, Stephen [0000-0003-1308-5755]
dc.contributor.orcidPathan, Nazima [0000-0002-9447-4252]
dc.identifier.eissn2044-6055
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMRC (MR/V006118/1)
pubs.funder-project-idAddenbrooke's Charitable Trust (ACT) (900240)
pubs.funder-project-idAction Medical Research (GN2903)
cam.issuedOnline2021-11-29
cam.depositDate2022-01-08
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International