This article analyzes the critical geopolitics of knowledge production in Brazil during the 20th century. It offers a critical appraisal of recent calls to decolonize political geography by locating the role played by actors and institutions in the Global South within the broader narrative of the Green Revolution. Historical accounts of the Green Revolution have only recently started to incorporate perspectives of and attribute agency to actors in the Global South. However, Brazil has largely been left out of the geographical scope of the Green Revolution. This article focuses on U.S.-Brazilian geopolitical relations behind an effort to reproduce the U.S. model of higher education, rural extension and agricultural research in Brazil. I argue that the confluence of Brazil's geopolitical importance with opportunities for foreign investment in its agricultural sector brought together U.S. and Brazilian experts and expertise to modernize Brazilian agriculture. The case of transnational soybean research illustrates the importance of this relationship in transforming Brazil's agricultural sector and limiting alternatives. This article offers an account of how the geopolitics of knowledge production shape the long-term institutional legacies of national research institutions.