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dc.contributor.authorKuett, L
dc.contributor.authorCatena, R
dc.contributor.authorÖzcan, A
dc.contributor.authorPlüss, A
dc.contributor.authorAli, HR
dc.contributor.authorSa’d, MA
dc.contributor.authorAlon, S
dc.contributor.authorAparicio, S
dc.contributor.authorBattistoni, G
dc.contributor.authorBalasubramanian, Shankar
dc.contributor.authorBecker, R
dc.contributor.authorBodenmiller, B
dc.contributor.authorBoyden, ES
dc.contributor.authorBressan, D
dc.contributor.authorBruna, A
dc.contributor.authorBurger, M
dc.contributor.authorCaldas, Carlos
dc.contributor.authorCallari, M
dc.contributor.authorCannell, IG
dc.contributor.authorCasbolt, H
dc.contributor.authorChornay, N
dc.contributor.authorCui, Y
dc.contributor.authorDariush, Aliakbar
dc.contributor.authorDinh, K
dc.contributor.authorEmenari, A
dc.contributor.authorEyal-Lubling, Y
dc.contributor.authorFan, J
dc.contributor.authorFatemi, A
dc.contributor.authorFisher, E
dc.contributor.authorGonzález-Solares, EA
dc.contributor.authorGonzález-Fernández, C
dc.contributor.authorGoodwin, D
dc.contributor.authorGreenwood, W
dc.contributor.authorGrimaldi, F
dc.contributor.authorHannon, GJ
dc.contributor.authorHarris, S
dc.contributor.authorJauset, C
dc.contributor.authorJoyce, JA
dc.contributor.authorKaragiannis, ED
dc.contributor.authorKovačević, T
dc.contributor.authorKunes, R
dc.contributor.authorYoldaş, AK
dc.contributor.authorLai, D
dc.contributor.authorLaks, E
dc.contributor.authorLee, H
dc.contributor.authorLee, M
dc.contributor.authorLerda, G
dc.contributor.authorLi, Y
dc.contributor.authorMcPherson, A
dc.contributor.authorMillar, N
dc.contributor.authorMulvey, CM
dc.contributor.authorNugent, I
dc.contributor.authorO’Flanagan, CH
dc.contributor.authorPaez-Ribes, M
dc.contributor.authorPearsall, I
dc.contributor.authorQosaj, F
dc.contributor.authorRoth, AJ
dc.contributor.authorRueda, OM
dc.contributor.authorRuiz, T
dc.contributor.authorSawicka, K
dc.contributor.authorSepúlveda, LA
dc.contributor.authorShah, SP
dc.contributor.authorShea, A
dc.contributor.authorSinha, A
dc.contributor.authorSmith, A
dc.contributor.authorTavaré, S
dc.contributor.authorTietscher, S
dc.contributor.authorVázquez-García, I
dc.contributor.authorVogl, SL
dc.contributor.authorWalton, NA
dc.contributor.authorWassie, AT
dc.contributor.authorWatson, SS
dc.contributor.authorWeselak, J
dc.contributor.authorWild, SA
dc.contributor.authorWilliams, E
dc.contributor.authorWindhager, J
dc.contributor.authorXia, C
dc.contributor.authorZheng, P
dc.contributor.authorZhuang, X
dc.contributor.authorSchraml, P
dc.contributor.authorMoch, H
dc.contributor.authorde Souza, N
dc.contributor.authorBodenmiller, B
dc.date.accessioned2022-01-13T00:31:12Z
dc.date.available2022-01-13T00:31:12Z
dc.date.issued2021-01-01
dc.identifier.issn2662-1347
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332671
dc.description.abstractA holistic understanding of tissue and organ structure and function requires the detection of molecular constituents in their original three-dimensional (3D) context. Imaging mass cytometry (IMC) enables simultaneous detection of up to 40 antigens and transcripts using metal-tagged antibodies but has so far been restricted to two-dimensional imaging. Here we report the development of 3D IMC for multiplexed 3D tissue analysis at single-cell resolution and demonstrate the utility of the technology by analysis of human breast cancer samples. The resulting 3D models reveal cellular and microenvironmental heterogeneity and cell-level tissue organization not detectable in two dimensions. 3D IMC will prove powerful in the study of phenomena occurring in 3D space such as tumor cell invasion and is expected to provide invaluable insights into cellular microenvironments and tissue architecture.
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleThree-dimensional imaging mass cytometry for highly multiplexed molecular and cellular mapping of tissues and the tumor microenvironment
dc.typeArticle
dc.publisher.departmentoffice of The School of Clinical Medicine
dc.publisher.departmentDepartment of Oncology
dc.publisher.departmentInstitute of Astronomy
dc.date.updated2022-01-11T11:12:28Z
prism.endingPage12
prism.publicationDate2021
prism.publicationNameNature Cancer
prism.startingPage1
dc.identifier.doi10.17863/CAM.80116
dcterms.dateAccepted2021-11-03
rioxxterms.versionofrecord10.1038/s43018-021-00301-w
rioxxterms.versionVoR
dc.contributor.orcidBalasubramanian, Shankar [0000-0002-0281-5815]
dc.contributor.orcidCaldas, Carlos [0000-0003-3547-1489]
dc.identifier.eissn2662-1347
rioxxterms.typeJournal Article/Review
pubs.funder-project-idCancer Research UK (unknown)
pubs.funder-project-idCancer Research UK (unknown)
pubs.funder-project-idCancer Research UK (unknown)
pubs.funder-project-idCancer Research UK (60098573)
pubs.funder-project-idCancer Research UK (unknown)
pubs.funder-project-idCancer Research UK (CB4140)
pubs.funder-project-idEuropean Research Council (694620)
pubs.funder-project-idCancer Research UK (CRUK-A9401)
pubs.funder-project-idCancer Research Uk (None)
pubs.funder-project-idCancer Research UK (C37096/A16673)
cam.issuedOnline2021-12-24
cam.depositDate2022-01-11
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International