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Three-dimensional imaging mass cytometry for highly multiplexed molecular and cellular mapping of tissues and the tumor microenvironment

Published version
Peer-reviewed

Type

Article

Change log

Authors

Kuett, L 
Catena, R 
Özcan, A 
Plüss, A 
Ali, HR 

Abstract

A holistic understanding of tissue and organ structure and function requires the detection of molecular constituents in their original three-dimensional (3D) context. Imaging mass cytometry (IMC) enables simultaneous detection of up to 40 antigens and transcripts using metal-tagged antibodies but has so far been restricted to two-dimensional imaging. Here we report the development of 3D IMC for multiplexed 3D tissue analysis at single-cell resolution and demonstrate the utility of the technology by analysis of human breast cancer samples. The resulting 3D models reveal cellular and microenvironmental heterogeneity and cell-level tissue organization not detectable in two dimensions. 3D IMC will prove powerful in the study of phenomena occurring in 3D space such as tumor cell invasion and is expected to provide invaluable insights into cellular microenvironments and tissue architecture.

Description

Keywords

Journal Title

Nature Cancer

Conference Name

Journal ISSN

2662-1347
2662-1347

Volume Title

Publisher

Springer Science and Business Media LLC
Sponsorship
Cancer Research UK (unknown)
Cancer Research UK (unknown)
Cancer Research UK (unknown)
Cancer Research UK (60098573)
Cancer Research UK (unknown)
Cancer Research UK (CB4140)
European Research Council (694620)
Cancer Research UK (CRUK-A9401)
Cancer Research Uk (None)
Cancer Research UK (C37096/A16673)