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dc.contributor.authorReint, Ganna
dc.contributor.authorLi, Zhuokun
dc.contributor.authorLabun, Kornel
dc.contributor.authorKeskitalo, Salla
dc.contributor.authorSoppa, Inkeri
dc.contributor.authorMamia, Katariina
dc.contributor.authorTolo, Eero
dc.contributor.authorSzymanska, Monika
dc.contributor.authorMeza-Zepeda, Leonardo A
dc.contributor.authorLorenz, Susanne
dc.contributor.authorCieslar-Pobuda, Artur
dc.contributor.authorHu, Xian
dc.contributor.authorBordin, Diana L
dc.contributor.authorStaerk, Judith
dc.contributor.authorValen, Eivind
dc.contributor.authorSchmierer, Bernhard
dc.contributor.authorVarjosalo, Markku
dc.contributor.authorTaipale, Anssi Jussi Nikolai
dc.contributor.authorHaapaniemi, Emma
dc.date.accessioned2022-01-13T00:31:28Z
dc.date.available2022-01-13T00:31:28Z
dc.date.issued2021-12-13
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332674
dc.description.abstractPrecision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR) to introduce custom DNA sequences to target sites. The HDR editing efficiency varies between cell types and genomic sites, and the sources of this variation are incompletely understood. Here, we have studied the effect of 450 DNA repair protein-Cas9 fusions on CRISPR genome editing outcomes. We find the majority of fusions to improve precision genome editing only modestly in a locus- and cell-type specific manner. We identify Cas9-POLD3 fusion that enhances editing by speeding up the initiation of DNA repair. We conclude that while DNA repair protein fusions to Cas9 can improve HDR CRISPR editing, most need to be optimized to the cell type and genomic site, highlighting the diversity of factors contributing to locus-specific genome editing outcomes.
dc.format.mediumPrint-Electronic
dc.publishereLife Sciences Publications, Ltd
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectcell biology
dc.titleRapid genome editing by CRISPR-Cas9-POLD3 fusion.
dc.typeArticle
dc.publisher.departmentDepartment of Biochemistry
dc.date.updated2022-01-11T16:08:22Z
prism.publicationDate2021
prism.publicationNameElife
prism.volume10
dc.identifier.doi10.17863/CAM.80119
dcterms.dateAccepted2021-11-15
rioxxterms.versionofrecord10.7554/eLife.75415
rioxxterms.versionVoR
dc.contributor.orcidReint, Ganna [0000-0003-4823-5485]
dc.contributor.orcidLi, Zhuokun [0000-0001-7297-6916]
dc.contributor.orcidSzymanska, Monika [0000-0003-0957-9568]
dc.contributor.orcidHu, Xian [0000-0002-3381-7514]
dc.contributor.orcidStaerk, Judith [0000-0001-8698-6998]
dc.contributor.orcidSchmierer, Bernhard [0000-0002-9082-7022]
dc.contributor.orcidVarjosalo, Markku [0000-0002-1340-9732]
dc.contributor.orcidTaipale, Anssi Jussi Nikolai [0000-0003-4204-0951]
dc.contributor.orcidHaapaniemi, Emma [0000-0002-6693-8208]
dc.identifier.eissn2050-084X
rioxxterms.typeJournal Article/Review
cam.issuedOnline2021-12-13
cam.depositDate2022-01-11
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International