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dc.contributor.authorOsma-Garcia, Ines C
dc.contributor.authorCapitan-Sobrino, Dunja
dc.contributor.authorMouysset, Mailys
dc.contributor.authorBell, Sarah Elizabeth
dc.contributor.authorLebeurrier, Manuel
dc.contributor.authorTurner, Martin
dc.contributor.authorDiaz-Muñoz, Manuel D
dc.date.accessioned2022-01-14T00:31:13Z
dc.date.available2022-01-14T00:31:13Z
dc.date.issued2021-11-12
dc.identifier.issn2041-1723
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332700
dc.description.abstractThe germinal centre (GC) is required for the generation of high affinity antibodies and immunological memory. Here we show that the RNA binding protein HuR has an essential function in GC B cells to sustain the GC response. In its absence, the GC reaction and production of high-affinity antibody is severely impaired. Mechanistically, HuR affects the transcriptome qualitatively and quantitatively. The expression and splicing patterns of hundreds of genes are altered in the absence of HuR. Among these genes, HuR is required for the expression of Myc and a Myc-dependent transcriptional program that controls GC B cell proliferation and Ig somatic hypermutation. Additionally, HuR regulates the splicing and abundance of mRNAs required for entry into and transition through the S phase of the cell cycle, and it modulates a gene signature associated with DNA deamination protecting GC B cells from DNA damage and cell death.
dc.format.mediumElectronic
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAnimals
dc.subjectCell Proliferation
dc.subjectComputational Biology
dc.subjectELAV-Like Protein 1
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectGerminal Center
dc.subjectMice
dc.subjectSequence Analysis, RNA
dc.titleThe RNA-binding protein HuR is required for maintenance of the germinal centre response.
dc.typeArticle
dc.publisher.departmentBabraham Institute
dc.date.updated2022-01-13T09:53:59Z
prism.issueIdentifier1
prism.number6556
prism.publicationDate2021
prism.publicationNameNat Commun
prism.startingPage6556
prism.volume12
dc.identifier.doi10.17863/CAM.80144
dcterms.dateAccepted2021-10-22
rioxxterms.versionofrecord10.1038/s41467-021-26908-2
rioxxterms.versionVoR
dc.contributor.orcidBell, Sarah Elizabeth [0000-0002-3249-707X]
dc.contributor.orcidTurner, Martin [0000-0002-3801-9896]
dc.contributor.orcidDiaz-Muñoz, Manuel D [0000-0002-9227-7474]
dc.identifier.eissn2041-1723
rioxxterms.typeJournal Article/Review
cam.issuedOnline2021-11-12
cam.depositDate2022-01-13
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International