Show simple item record

dc.contributor.authorCai, Shenglin
dc.contributor.authorSze, Jasmine YY
dc.contributor.authorIvanov, Aleksandar P
dc.contributor.authorEdel, Joshua B
dc.date.accessioned2022-01-14T00:31:17Z
dc.date.available2022-01-14T00:31:17Z
dc.date.issued2019-04-17
dc.identifier.issn2041-1723
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332701
dc.description.abstractThe identification of short nucleic acids and proteins at the single molecule level is a major driving force for the development of novel detection strategies. Nanopore sensing has been gaining in prominence due to its label-free operation and single molecule sensitivity. However, it remains challenging to detect small molecules selectively. Here we propose to combine the electrical sensing modality of a nanopore with fluorescence-based detection. Selectivity is achieved by grafting either molecular beacons, complementary DNA, or proteins to a DNA molecular carrier. We show that the fraction of synchronised events between the electrical and optical channels, can be used to perform single molecule binding assays without the need to directly label the analyte. Such a strategy can be used to detect targets in complex biological fluids such as human serum and urine. Future optimisation of this technology may enable novel assays for quantitative protein detection as well as gene mutation analysis with applications in next-generation clinical sample analysis.
dc.format.mediumElectronic
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBiosensing Techniques
dc.subjectDNA
dc.subjectFluorescence
dc.subjectHumans
dc.subjectNanopores
dc.subjectNanotechnology
dc.subjectProteins
dc.titleSmall molecule electro-optical binding assay using nanopores.
dc.typeArticle
dc.publisher.departmentDepartment of Chemistry
dc.date.updated2022-01-13T10:06:32Z
prism.issueIdentifier1
prism.numberARTN 1797
prism.publicationDate2019
prism.publicationNameNat Commun
prism.startingPage1797
prism.volume10
dc.identifier.doi10.17863/CAM.80145
dcterms.dateAccepted2019-03-12
rioxxterms.versionofrecord10.1038/s41467-019-09476-4
rioxxterms.versionVoR
dc.contributor.orcidCai, Shenglin [0000-0002-7815-6406]
dc.contributor.orcidSze, Jasmine YY [0000-0001-9663-0194]
dc.contributor.orcidIvanov, Aleksandar P [0000-0003-1419-1381]
dc.contributor.orcidEdel, Joshua B [0000-0001-5870-8659]
dc.identifier.eissn2041-1723
rioxxterms.typeJournal Article/Review
cam.issuedOnline2019-04-17
cam.depositDate2022-01-13
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International