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dc.contributor.authorHuang, Ni
dc.contributor.authorSeow, Wei Qiang
dc.contributor.authorAppert, Alex
dc.contributor.authorDong, Yan
dc.contributor.authorStempor, Przemyslaw
dc.contributor.authorAhringer, Julie
dc.date.accessioned2022-01-21T00:30:09Z
dc.date.available2022-01-21T00:30:09Z
dc.date.issued2022-02
dc.identifier.issn1088-9051
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332815
dc.description.abstractNuclear organization and chromatin interactions are important for genome function, yet determining chromatin connections at high resolution remains a major challenge. To address this, we developed Accessible Region Conformation Capture (ARC-C), which profiles interactions between regulatory elements genome-wide without a capture step. Applied to Caenorhabditis elegans, ARC-C identifies approximately 15,000 significant interactions between regulatory elements at 500-bp resolution. Of 105 TFs or chromatin regulators tested, we find that the binding sites of 60 are enriched for interacting with each other, making them candidates for mediating interactions. These include cohesin and condensin II. Applying ARC-C to a mutant of transcription factor BLMP-1 detected changes in interactions between its targets. ARC-C simultaneously profiles domain-level architecture, and we observe that C. elegans chromatin domains defined by either active or repressive modifications form topologically associating domains (TADs) that interact with A/B (active/inactive) compartment-like structure. Furthermore, we discover that inactive compartment interactions are dependent on H3K9 methylation. ARC-C is a powerful new tool to interrogate genome architecture and regulatory interactions at high resolution.
dc.format.mediumPrint-Electronic
dc.publisherCold Spring Harbor Laboratory
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleAccessible Region Conformation Capture (ARC-C) gives high-resolution insights into genome architecture and regulation.
dc.typeArticle
dc.publisher.departmentWellcome Trust/Cancer Research Uk Gurdon Institute
dc.publisher.departmentDepartment of Genetics
dc.date.updated2022-01-18T20:52:08Z
prism.endingPagegr.275669.121
prism.publicationDate2021
prism.publicationNameGenome Res
prism.startingPagegr.275669.121
dc.identifier.doi10.17863/CAM.80249
dcterms.dateAccepted2021-12-19
rioxxterms.versionofrecord10.1101/gr.275669.121
rioxxterms.versionVoR
dc.contributor.orcidStempor, Przemyslaw [0000-0002-9464-7475]
dc.contributor.orcidAhringer, Julie [0000-0002-7074-4051]
dc.identifier.eissn1549-5469
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (101863/Z/13/Z)
pubs.funder-project-idWellcome Trust (203144/Z/16/Z)
pubs.funder-project-idWellcome Trust (092096/Z/10/Z)
pubs.funder-project-idCancer Research Uk (None)
pubs.funder-project-idMRC (MR/S021620/1)
cam.issuedOnline2021-12-21
cam.depositDate2022-01-18
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International