Comparative chromosome painting in Spizaetus tyrannus and Gallus gallus with the use of macro- and microchromosome probes.
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Authors
Carvalho, Carlos A
Furo, Ivanete O
O'Brien, Patricia CM
Pereira, Jorge
O'Connor, Rebeca E
Griffin, Darren
Ferguson-Smith, Malcolm
Publication Date
2021Journal Title
PLoS One
ISSN
1932-6203
Publisher
Public Library of Science (PLoS)
Volume
16
Issue
11
Pages
e0259905
Type
Article
This Version
VoR
Physical Medium
Electronic-eCollection
Metadata
Show full item recordCitation
Carvalho, C. A., Furo, I. O., O'Brien, P. C., Pereira, J., O'Connor, R. E., Griffin, D., Ferguson-Smith, M., & et al. (2021). Comparative chromosome painting in Spizaetus tyrannus and Gallus gallus with the use of macro- and microchromosome probes.. PLoS One, 16 (11), e0259905. https://doi.org/10.1371/journal.pone.0259905
Abstract
Although most birds show karyotypes with diploid number (2n) around 80, with few macrochromosomes and many microchromosomes pairs, some groups, such as the Accipitriformes, are characterized by a large karyotypic reorganization, which resulted in complements with low diploid numbers, and a smaller number of microchromosomal pairs when compared to other birds. Among Accipitriformes, the Accipitridae family is the most diverse and includes, among other subfamilies, the subfamily Aquilinae, composed of medium to large sized species. The Black-Hawk-Eagle (Spizaetus tyrannus-STY), found in South America, is a member of this subfamily. Available chromosome data for this species includes only conventional staining. Hence, in order to provide additional information on karyotype evolution process within this group, we performed comparative chromosome painting between S. tyrannus and Gallus gallus (GGA). Our results revealed that at least 29 fission-fusion events occurred in the STY karyotype, based on homology with GGA. Fissions occurred mainly in syntenic groups homologous to GGA1-GGA5. On the other hand, the majority of the microchromosomes were found fused to other chromosomal elements in STY, indicating these rearrangements played an important role in the reduction of the 2n to 68. Comparison with hybridization pattern of the Japanese-Mountain-Eagle (Nisaetus nipalensis orientalis), the only Aquilinae analyzed by comparative chromosome painting previously, did not reveal any synapomorphy that could represent a chromosome signature to this subfamily. Therefore, conclusions about karyotype evolution in Aquilinae require additional painting studies.
Keywords
Animals, Cells, Cultured, Chickens, Chromosome Painting, Chromosomes, Artificial, Bacterial, DNA Probes, Evolution, Molecular, Female, Gene Fusion, In Situ Hybridization, Fluorescence, Karyotype, Phylogeography, Raptors
Identifiers
External DOI: https://doi.org/10.1371/journal.pone.0259905
This record's URL: https://www.repository.cam.ac.uk/handle/1810/332829
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