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dc.contributor.authorShepherd, Jennifer H
dc.contributor.authorHoward, Daniel
dc.contributor.authorWaller, Amie
dc.contributor.authorFoster, Holly Rebecca
dc.contributor.authorMuller, Annett
dc.contributor.authorMoreau, Thomas
dc.contributor.authorEvans, Amanda L
dc.contributor.authorArumugam, Meera
dc.contributor.authorBouët Chalon, Guénaëlle
dc.contributor.authorVriend, Eleonora
dc.contributor.authorDavidenko, Natalia
dc.contributor.authorGhevaert, Cedric
dc.contributor.authorBest, Serena
dc.contributor.authorCameron, Ruth
dc.date.accessioned2022-01-21T17:48:46Z
dc.date.available2022-01-21T17:48:46Z
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332861
dc.descriptionPlatelet transfusions are a key treatment option for a range of life threatening conditions including cancer, chemotherapy and surgery. Efficient ex vivo systems to generate donor independent platelets in clinically relevant numbers could provide a useful substitute. Large quantities of megakaryocytes (MKs) can be produced from human pluripotent stem cells, but in 2D culture the ratio of platelets harvested from MK cells has been limited and restricts production rate. The development of biomaterial cell supports that replicate vital hematopoietic micro-environment cues are one strategy that may increase in vitro platelet production rates from iPS derived Megakaryocyte cells. In this paper, we present the results obtained generating, simulating and using a novel structurally-graded collagen scaffold within a flow bioreactor system seeded with programmed stem cells. Theoretical analysis of porosity using micro-computed tomography analysis and synthetic micro-particle filtration provided a predictive tool to tailor cell distribution throughout the material. When used with MK programmed stem cells the graded scaffolds influenced cell location while maintaining the ability to continuously release metabolically active CD41+ CD42+ functional platelets. This scaffold design and novel fabrication technique offers a significant advance in understanding the influence of scaffold architectures on cell seeding, retention and platelet production.
dc.formatMicrosoft Office Excel, Microsoft Office Word
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectscaffold architecture
dc.subjectbioreactor
dc.subjectParallel flow membrane filter
dc.subjectcollagen
dc.subjectbone marrow model
dc.subjectdepth straining
dc.subjectinterconnectivity
dc.subjectsettling zones
dc.subjectshear flow
dc.subjectHuman Induced Pluripotent Stem Cells
dc.subjectForward programming
dc.subjectMegakaryocytes
dc.subjectplatelets
dc.titleResearch data supporting 'Structurally graduated collagen scaffolds applied to the ex vivo generation of platelets from human pluripotent stem cell-derived megakaryocytes: enhancing production and purity'
dc.typeDataset
dc.identifier.doi10.17863/CAM.26141
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
datacite.contributor.supervisorCameron, Ruth Elizabeth
dcterms.format.xlsx, .csv .pdf
dc.contributor.orcidMoreau, Thomas [0000-0003-1090-6685]
dc.contributor.orcidGhevaert, Cedric [0000-0002-9251-0934]
dc.contributor.orcidBest, Serena [0000-0001-7866-8607]
dc.contributor.orcidCameron, Ruth [0000-0003-1573-4923]
rioxxterms.typeOther
pubs.funder-project-idEuropean Research Council (320598)
datacite.issupplementto.doi10.1016/j.biomaterials.2018.08.019
datacite.issupplementto.urlhttps://www.repository.cam.ac.uk/handle/1810/284878


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)