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dc.contributor.authorPerera, Marianne R
dc.contributor.authorRoche, Kathryn L
dc.contributor.authorMurphy, Eain A
dc.contributor.authorSinclair, John
dc.date.accessioned2022-01-25T00:30:54Z
dc.date.available2022-01-25T00:30:54Z
dc.date.issued2022-01-26
dc.identifier.issn1999-4915
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332901
dc.description.abstractLong non-coding RNA β2.7 is the most highly transcribed viral gene during latent human cytomegalovirus (HCMV) infection. However, as yet, no function has ever been ascribed to β2.7 during HCMV latency. Here we show that β2.7 protects against apoptosis induced by high levels of reactive oxygen species (ROS) in infected monocytes, which routinely support latent HCMV infection. Monocytes infected with a wild-type (WT) virus, but not virus deleted for the β2.7 gene (Δβ2.7), are protected against mitochondrial stress and subsequent apoptosis. Protected monocytes display lower levels of ROS and additionally, stress-induced death in the absence of β2.7 can be reversed by an antioxidant which reduces ROS levels. Furthermore, we show that infection with WT but not Δβ2.7 virus results in strong upregulation of a cellular antioxidant enzyme, superoxide dismutase 2 (SOD2) in CD14+ monocytes. These observations identify a role for the β2.7 viral transcript, the most abundantly expressed viral RNA during latency but for which no latency-associated function has ever been ascribed, and demonstrate a novel way in which HCMV protects infected monocytes from pro-death signals to optimise latent carriage.
dc.description.sponsorshipMRC
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleA Viral Long Non-Coding RNA Protects against Cell Death during Human Cytomegalovirus Infection of CD14+ Monocytes.
dc.typeArticle
dc.publisher.departmentDepartment of Medicine
dc.date.updated2022-01-24T08:21:41Z
prism.publicationNameViruses
dc.identifier.doi10.17863/CAM.80331
dcterms.dateAccepted2022-01-21
rioxxterms.versionofrecord10.3390/v14020246
rioxxterms.versionAM
dc.contributor.orcidPerera, Marianne R [0000-0001-9175-0568]
dc.contributor.orcidSinclair, John [0000-0002-2616-9571]
dc.identifier.eissn1999-4915
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (073979/Z/03/A)
pubs.funder-project-idMedical Research Council (MR/S00081X/1)
cam.orpheus.successTue Feb 01 19:02:46 GMT 2022 - Embargo updated
cam.depositDate2022-01-24
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement
rioxxterms.freetoread.startdate2022-01-26


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International