Dynamic shapes of the zygote and two-cell mouse and human.
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Authors
Vincent, Anna
Jones, Celine
Coward, Kevin
Publication Date
2021-12-15Journal Title
Biol Open
ISSN
2046-6390
Publisher
The Company of Biologists
Volume
10
Issue
12
Number
ARTN bio059013
Pages
bio059013
Type
Article
This Version
VoR
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Graham, C. F., Windsor, S., Ajduk, A., Trinh, T., Vincent, A., Jones, C., Coward, K., et al. (2021). Dynamic shapes of the zygote and two-cell mouse and human.. Biol Open, 10 (12. ARTN bio059013), bio059013. https://doi.org/10.1242/bio.059013
Abstract
Mouse zygote morphokinetics were measured during interphase, the mitotic period, cytokinesis, and two-cell stage. Sequences of rounder-distorted-rounder shapes were revealed, as were changing patterns of cross section area. A calcium chelator and an actin-disrupting agent inhibited the area changes that occurred between pronuclear envelope breakdown and cytokinesis. During cell division, two vortices developed in each nascent cell and they rotated in opposite directions at each end of the cell, a pattern that sometimes persisted for up to 10 h. Exchange with the environment may have been promoted by these shape and area cycles and persisting circulation in the cytoplasm may have a similar function between a cell's interior and periphery. Some of these movements were sporadically also seen in human zygotes with abnormal numbers of pronuclei and the two-cell stages that developed from these compromised human zygotes.
Keywords
Cytoplasm vortices, Human, Morphokinetics, Mouse zygote, Pronuclear fading, Shape cycles
Identifiers
External DOI: https://doi.org/10.1242/bio.059013
This record's URL: https://www.repository.cam.ac.uk/handle/1810/332979
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