Rapid genome editing by CRISPR-Cas9-POLD3 fusion
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Abstract
Precision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR) to introduce custom DNA sequences to target sites. The HDR editing efficiency varies between cell types and genomic sites, and the sources of this variation are incompletely understood. Here, we have studied the effect of 450 DNA repair protein-Cas9 fusions on CRISPR genome editing outcomes. We find the majority of fusions to improve precision genome editing only modestly in a locus- and cell-type specific manner. We identify Cas9-POLD3 fusion that enhances editing by speeding up the initiation of DNA repair. We conclude that while DNA repair protein fusions to Cas9 can improve HDR CRISPR editing, most need to be optimized to the cell type and genomic site, highlighting the diversity of factors contributing to locus-specific genome editing outcomes.
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Funder: Barncancerfonden; FundRef: http://dx.doi.org/10.13039/501100006313
Funder: Norwegian Research Council; FundRef: http://dx.doi.org/10.13039/501100005416
Funder: Knut och Alice Wallenbergs Stiftelse; FundRef: http://dx.doi.org/10.13039/501100004063
Funder: Cancerfonden; FundRef: http://dx.doi.org/10.13039/501100002794
Funder: Instrumentariumin Tiedesäätiö; FundRef: http://dx.doi.org/10.13039/501100008413
Funder: Science for Life Laboratory; FundRef: http://dx.doi.org/10.13039/501100009252
Funder: Academy of Finland; FundRef: http://dx.doi.org/10.13039/501100002341