jats:pThere is a gap in understanding the relationships between the transformation of agricultural landscapes, ecosystem services and human well-being in the peri-urban fringe of major cities worldwide. In this paper, we use semi-structured interviews, perception surveys, social surveys and field mapping to examine linkages between agricultural and landscape transition, ecosystem services and human well-being in five sample villages in Xi’an metropolitan zone, China. The results indicate that: (1) Agricultural change has increased landscape fragmentation, with a shift from grain to more profitable horticulture and nursery production. The farming system is more diversified and exhibits a multifunctional character. (2) This transformation has had a significant impact on the character of the agroecosystem. (3) The agricultural transformation towards greater multifunctionality has increased the supply of ecosystem services, including tourism-related activities, potentially improving human well-being. (4) Different combinations of activities in the sample villages were evaluated with respect to a well-being index, indicating the importance of combining horticulture and tourism. (5) Linkages identified between agricultural transformation, ecosystem services and human well-being may have significant implications for potential approaches within future studies.</jats:p>