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dc.contributor.authorBartas, Martin
dc.contributor.authorSlychko, Kristyna
dc.contributor.authorBrázda, Václav
dc.contributor.authorČerveň, Jiří
dc.contributor.authorBeaudoin, Christopher
dc.contributor.authorBlundell, Tom
dc.contributor.authorPečinka, Petr
dc.date.accessioned2022-01-28T14:42:06Z
dc.date.available2022-01-28T14:42:06Z
dc.date.issued2022-01-11
dc.identifier.issn1661-6596
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/333042
dc.description.abstractZ-DNA and Z-RNA are functionally important left-handed structures of nucleic acids, which play a significant role in several molecular and biological processes including DNA replication, gene expression regulation and viral nucleic acid sensing. Most proteins that have been proven to interact with Z-DNA/Z-RNA contain the so-called Zα domain, which is structurally well conserved. To date, only eight proteins with Zα domain have been described within a few organisms (including human, mouse, Danio rerio, Trypanosoma brucei and some viruses). Therefore, this paper aimed to search for new Z-DNA/Z-RNA binding proteins in the complete PDB structures database and from the AlphaFold2 protein models. A structure-based similarity search found 14 proteins with highly similar Zα domain structure in experimentally-defined proteins and 185 proteins with a putative Zα domain using the AlphaFold2 models. Structure-based alignment and molecular docking confirmed high functional conservation of amino acids involved in Z-DNA/Z-RNA, suggesting that Z-DNA/Z-RNA recognition may play an important role in a variety of cellular processes.
dc.languageen
dc.publisherMDPI AG
dc.subjectZ-DNA
dc.subjectZ-RNA
dc.subjectZα domain
dc.subjectprotein binding
dc.subjectbioinformatics
dc.titleSearching for New Z-DNA/Z-RNA Binding Proteins Based on Structural Similarity to Experimentally Validated Zα Domain.
dc.typeOther
dc.date.updated2022-01-28T14:42:05Z
prism.issueIdentifier2
prism.publicationNameInt J Mol Sci
prism.volume23
dc.identifier.doi10.17863/CAM.80466
dcterms.dateAccepted2022-01-05
rioxxterms.versionofrecord10.3390/ijms23020768
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidBartas, Martin [0000-0002-4415-2220]
dc.contributor.orcidBrázda, Václav [0000-0003-2837-4226]
dc.contributor.orcidBlundell, Tom [0000-0002-2708-8992]
dc.contributor.orcidPečinka, Petr [0000-0003-1270-3646]
dc.identifier.eissn1422-0067
cam.issuedOnline2022-01-11


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