Show simple item record

dc.contributor.authorZhu, Jinbo
dc.contributor.authorYan, Zhiqiang
dc.contributor.authorBošković, Filip
dc.contributor.authorHaynes, Cally JE
dc.contributor.authorKieffer, Marion
dc.contributor.authorGreenfield, Jake L
dc.contributor.authorWang, Jin
dc.contributor.authorNitschke, Jonathan R
dc.contributor.authorKeyser, Ulrich F
dc.date.accessioned2022-01-28T14:44:59Z
dc.date.available2022-01-28T14:44:59Z
dc.date.issued2021-11-10
dc.identifier.issn2041-6520
dc.identifier.other34881008
dc.identifier.otherPMC8580047
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/333080
dc.description.abstractSince the discovery of the G-quadruplex (G4) structure in telomeres in 1980s, studies have established the role it plays in various biological processes. Here we report binding between DNA G4 and a self-assembled tetrahedral metal-organic cage 1 and consequent formation of aggregates, whereby the cage protects the DNA G4 from cleavage by S1 nuclease. We monitor DNA-cage interaction using fluorescence spectroscopy, firstly by quenching of a fluorescent label appended to the 5' end of G4. Secondly, we detect the decrease in fluorescence of the G4-selective dyes thioflavin-T and Zn-PPIX bound to various DNA G4 sequences following the addition of cage 1. Our results demonstrate that 1 interacts with a wide range of G4s. Moreover, gel electrophoresis, circular dichroism and dynamic light scattering measurements establish the binding of 1 to G4 and indicate the formation of aggregate structures. Finally, we find that DNA G4 contained in an aggregate of cage 1 is protected from cleavage by S1 nuclease.
dc.languageeng
dc.publisherRoyal Society of Chemistry (RSC)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcenlmid: 101545951
dc.sourceessn: 2041-6539
dc.titleFeII 4L4 tetrahedron binds and aggregates DNA G-quadruplexes.
dc.typeArticle
dc.date.updated2022-01-28T14:44:58Z
prism.endingPage14569
prism.issueIdentifier43
prism.publicationNameChem Sci
prism.startingPage14564
prism.volume12
dc.identifier.doi10.17863/CAM.80504
dcterms.dateAccepted2021-10-05
rioxxterms.versionofrecord10.1039/d1sc04430c
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidZhu, Jinbo [0000-0003-4935-825X]
dc.contributor.orcidYan, Zhiqiang [0000-0003-3865-3229]
dc.contributor.orcidBošković, Filip [0000-0001-7663-2408]
dc.contributor.orcidHaynes, Cally JE [0000-0003-4262-8560]
dc.contributor.orcidKieffer, Marion [0000-0003-0202-7445]
dc.contributor.orcidGreenfield, Jake L [0000-0002-7650-5414]
dc.contributor.orcidWang, Jin [0000-0002-2841-4913]
dc.contributor.orcidNitschke, Jonathan R [0000-0002-4060-5122]
dc.contributor.orcidKeyser, Ulrich F [0000-0003-3188-5414]
dc.identifier.eissn2041-6539
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/M008258/1)
pubs.funder-project-idEuropean Research Council (647144)
cam.issuedOnline2021-10-07


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International