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dc.contributor.authorBarrett, Nicholas J
dc.contributor.authorThyrring, Jakob
dc.contributor.authorHarper, Elizabeth M
dc.contributor.authorSejr, Mikael K
dc.contributor.authorSørensen, Jesper G
dc.contributor.authorPeck, Lloyd S
dc.contributor.authorClark, Melody S
dc.date.accessioned2022-01-28T16:33:16Z
dc.date.available2022-01-28T16:33:16Z
dc.date.issued2022-01-15
dc.identifier.issn2073-4425
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/333128
dc.description.abstractIncreases in Arctic temperatures have accelerated melting of the Greenland icesheet, exposing intertidal organisms, such as the blue mussel Mytilus edulis, to high air temperatures and low salinities in summer. However, the interaction of these combined stressors is poorly described at the transcriptional level. Comparing expression profiles of M. edulis from experimentally warmed (30 °C and 33 °C) animals kept at control (23‱) and low salinities (15‱) revealed a significant lack of enrichment for Gene Ontology terms (GO), indicating that similar processes were active under all conditions. However, there was a progressive increase in the abundance of upregulated genes as each stressor was applied, with synergistic increases at 33 °C and 15‱, suggesting combined stressors push the animal towards their tolerance thresholds. Further analyses comparing the effects of salinity alone (23‱, 15‱ and 5‱) showed high expression of stress and osmoregulatory marker genes at the lowest salinity, implying that the cell is carrying out intracellular osmoregulation to maintain the cytosol as hyperosmotic. Identification of aquaporins and vacuolar-type ATPase transcripts suggested the cell may use fluid-filled cavities to excrete excess intracellular water, as previously identified in embryonic freshwater mussels. These results indicate that M. edulis has considerable resilience to heat stress and highly efficient mechanisms to acclimatise to lowered salinity in a changing world.
dc.languageen
dc.publisherMDPI AG
dc.subjectblue mussel
dc.subjectcellular stress response
dc.subjectsalinity
dc.subjectthermal tolerance
dc.subjecttranscriptome
dc.subjectacclimation
dc.subjectfreshening
dc.subjectclimate change
dc.subjectaquaporins
dc.titleMolecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience.
dc.typeArticle
dc.date.updated2022-01-28T16:33:16Z
prism.issueIdentifier1
prism.publicationNameGenes (Basel)
prism.volume13
dc.identifier.doi10.17863/CAM.80551
dcterms.dateAccepted2022-01-13
rioxxterms.versionofrecord10.3390/genes13010155
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidBarrett, Nicholas J [0000-0003-1920-9704]
dc.contributor.orcidSørensen, Jesper G [0000-0002-9149-3626]
dc.contributor.orcidPeck, Lloyd S [0000-0003-3479-6791]
dc.identifier.eissn2073-4425
cam.issuedOnline2022-01-15


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