Current Methods and Pipelines for Image-Based Quantitation of Nuclear Shape and Nuclear Envelope Abnormalities.
Publication Date
2022-01-20Journal Title
Cells
ISSN
2073-4409
Publisher
MDPI AG
Volume
11
Issue
3
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Janssen, A., Breusegem, S., & Larrieu, D. (2022). Current Methods and Pipelines for Image-Based Quantitation of Nuclear Shape and Nuclear Envelope Abnormalities.. Cells, 11 (3) https://doi.org/10.3390/cells11030347
Abstract
Any given cell type has an associated "normal" nuclear morphology, which is important to maintain proper cellular functioning and safeguard genomic integrity. Deviations from this can be indicative of diseases such as cancer or premature aging syndrome. To accurately assess nuclear abnormalities, it is important to use quantitative measures of nuclear morphology. Here, we give an overview of several nuclear abnormalities, including micronuclei, nuclear envelope invaginations, blebs and ruptures, and review the current methods used for image-based quantification of these abnormalities. We discuss several parameters that can be used to quantify nuclear shape and compare their outputs using example images. In addition, we present new pipelines for quantitative analysis of nuclear blebs and invaginations. Quantitative analyses of nuclear aberrations and shape will be important in a wide range of applications, from assessments of cancer cell anomalies to studies of nucleus deformability under mechanical or other types of stress.
Keywords
nuclear envelope, nuclear envelope abnormality, lamina, nuclear morphology
Sponsorship
FEBS Long-Term Fellowship.
Funder references
Wellcome Trust (206242/Z/17/Z)
Federation of the European Biochemical Societies (FEBS) (Anne Janssen FEBS LTF)
Identifiers
External DOI: https://doi.org/10.3390/cells11030347
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333231
Rights
Licence:
https://creativecommons.org/licenses/by/4.0/
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