Cancer cell migration on straight, wavy, loop and grid microfibre patterns.
Authors
Piano, Nicholas
Cozens, Edward Jonathan
Dong, Lingqing
Publication Date
2022-01-25Journal Title
Biofabrication
ISSN
1758-5082
Publisher
IOP Publishing
Volume
14
Issue
2
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Zhang, D., Sheng, Y., Piano, N., Jakuszeit, T., Cozens, E. J., Dong, L., Buell, A. K., et al. (2022). Cancer cell migration on straight, wavy, loop and grid microfibre patterns.. Biofabrication, 14 (2) https://doi.org/10.1088/1758-5090/ac48e6
Abstract
Cell migration plays an important role in physiological and pathological processes where the fibrillar morphology of extracellular matrices (ECM) could regulate the migration dynamics. To mimic the morphological characteristics of fibrillar matrix structures, low-voltage continuous electrospinning was adapted to construct straight, wavy, looped and gridded fibre patterns made of polystyrene (of fibre diameter ca. 3μm). Cells were free to explore their different shapes in response to the directly-adhered fibre, as well as to the neighbouring patterns. For all the patterns studied, analysing cellular migration dynamics of MDA-MB-231 (a highly migratory breast cancer cell line) demonstrated two interesting findings: first, although cells dynamically adjust their shapes and migration trajectories in response to different fibrillar environments, their average step speed is minimally affected by the fibre global pattern; secondly, a switch in behaviour was observed when the pattern features approach the upper limit of the cell body's minor axis, reflecting that cells' ability to divert from an existing fibre track is limited by the size along the cell body's minor axis. It is therefore concluded that the upper limit of cell body's minor axis might act as a guide for the design of microfibre patterns for different purposes of cell migration.
Keywords
Paper, Placing 3D Bioprinting into the Context of Human Disease Modeling, breast cancer, cell migration, extracellular matrix, topography, pattern
Sponsorship
ERC H2020
Funder references
European Research Council (758865)
Identifiers
bfac48e6, ac48e6, bf-103450.r2
External DOI: https://doi.org/10.1088/1758-5090/ac48e6
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333336
Rights
Licence:
http://creativecommons.org/licenses/by/4.0
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