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dc.contributor.authorZhang, Duo
dc.contributor.authorSheng, Yaqi
dc.contributor.authorPiano, Nicholas
dc.contributor.authorJakuszeit, Theresa
dc.contributor.authorCozens, Edward Jonathan
dc.contributor.authorDong, Lingqing
dc.contributor.authorBuell, Alexander K
dc.contributor.authorPollet, Andreas
dc.contributor.authorLei, Iek Man
dc.contributor.authorWang, Wenyu
dc.contributor.authorTerentjev, Eugene
dc.contributor.authorHuang, Yan Yan Shery
dc.date.accessioned2022-01-28T16:48:25Z
dc.date.available2022-01-28T16:48:25Z
dc.date.issued2022-01-25
dc.date.submitted2021-08-22
dc.identifier.issn1758-5082
dc.identifier.otherbfac48e6
dc.identifier.otherac48e6
dc.identifier.otherbf-103450.r2
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/333336
dc.description.abstractCell migration plays an important role in physiological and pathological processes where the fibrillar morphology of extracellular matrices (ECM) could regulate the migration dynamics. To mimic the morphological characteristics of fibrillar matrix structures, low-voltage continuous electrospinning was adapted to construct straight, wavy, looped and gridded fibre patterns made of polystyrene (of fibre diameter ca. 3μm). Cells were free to explore their different shapes in response to the directly-adhered fibre, as well as to the neighbouring patterns. For all the patterns studied, analysing cellular migration dynamics of MDA-MB-231 (a highly migratory breast cancer cell line) demonstrated two interesting findings: first, although cells dynamically adjust their shapes and migration trajectories in response to different fibrillar environments, their average step speed is minimally affected by the fibre global pattern; secondly, a switch in behaviour was observed when the pattern features approach the upper limit of the cell body's minor axis, reflecting that cells' ability to divert from an existing fibre track is limited by the size along the cell body's minor axis. It is therefore concluded that the upper limit of cell body's minor axis might act as a guide for the design of microfibre patterns for different purposes of cell migration.
dc.description.sponsorshipERC H2020
dc.languageen
dc.publisherIOP Publishing
dc.subjectPaper
dc.subjectPlacing 3D Bioprinting into the Context of Human Disease Modeling
dc.subjectbreast cancer
dc.subjectcell migration
dc.subjectextracellular matrix
dc.subjecttopography
dc.subjectpattern
dc.titleCancer cell migration on straight, wavy, loop and grid microfibre patterns.
dc.typeArticle
dc.date.updated2022-01-28T16:48:24Z
prism.issueIdentifier2
prism.publicationNameBiofabrication
prism.volume14
dc.identifier.doi10.17863/CAM.80759
dcterms.dateAccepted2022-01-06
rioxxterms.versionofrecord10.1088/1758-5090/ac48e6
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0
dc.contributor.orcidZhang, Duo [0000-0002-7737-9229]
dc.contributor.orcidSheng, Yaqi [0000-0002-3545-6601]
dc.contributor.orcidJakuszeit, Theresa [0000-0003-3848-7486]
dc.contributor.orcidBuell, Alexander K [0000-0003-1161-3622]
dc.contributor.orcidPollet, Andreas [0000-0002-9571-2902]
dc.contributor.orcidLei, Iek Man [0000-0002-6337-1592]
dc.contributor.orcidWang, Wenyu [0000-0001-6580-8236]
dc.contributor.orcidTerentjev, Eugene [0000-0003-3517-6578]
dc.contributor.orcidHuang, Yan Yan Shery [0000-0003-2619-730X]
dc.identifier.eissn1758-5090
pubs.funder-project-idEuropean Research Council (758865)
cam.issuedOnline2022-01-25


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