A hexa-species transcriptome atlas of mammalian embryogenesis delineates metabolic regulation across three different implantation modes
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Publication Date
2022-06-16Journal Title
Nature Communications
ISSN
2041-1723
Publisher
Nature Research
Type
Article
This Version
VoR
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Malkowska, A., Penfold, C., Bergmann, S., & Boroviak, T. (2022). A hexa-species transcriptome atlas of mammalian embryogenesis delineates metabolic regulation across three different implantation modes. Nature Communications https://doi.org/10.1038/s41467-022-30194-x
Abstract
Mammalian embryogenesis relies on glycolysis and oxidative phosphorylation to balance the generation of biomass with energy production. However, the dynamics of metabolic regulation in the postimplantation embryo in vivo have remained elusive due to the inaccessibility of the implanted conceptus for biochemical studies. To address this issue, we compiled single-cell embryo profiling data in six mammalian species and determined their metabolic dynamics through glycolysis and oxidative phosphorylation associated gene expression. Strikingly, we identify a conserved switch from bivalent respiration in the late blastocyst towards a glycolytic metabolism in early gastrulation stages across species, which is independent of embryo implantation. Extraembryonic lineages followed the dynamics of the embryonic lineage, except visceral endoderm. Finally, we demonstrate that in vitro primate embryo culture substantially impacts metabolic gene regulation by comparison to in vivo samples. Our work reveals a conserved metabolic programme despite different implantation modes and highlights the need to optimise postimplantation embryo culture protocols.
Sponsorship
Wellcome Trust (206684/Z/17/Z)
Identifiers
External DOI: https://doi.org/10.1038/s41467-022-30194-x
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333399
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