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dc.contributor.authorPogodalla, Nicole
dc.contributor.authorKranenburg, Holger
dc.contributor.authorRey, Simone
dc.contributor.authorRodrigues, Silke
dc.contributor.authorCardona, Albert
dc.contributor.authorKlämbt, Christian
dc.date.accessioned2022-01-31T16:21:11Z
dc.date.available2022-01-31T16:21:11Z
dc.date.issued2021-11-04
dc.date.submitted2020-11-18
dc.identifier.others41467-021-26462-x
dc.identifier.other26462
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/333471
dc.description.abstractAbstract: In the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute to the formation of an internal diffusion barrier. We find that ensheathing glia are polarized with a basolateral plasma membrane rich in phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and the Na+/K+-ATPase Nervana2 (Nrv2) that abuts an extracellular matrix formed at neuropil-cortex interface. The apical plasma membrane is facing the neuropil and is rich in phosphatidylinositol-(4,5)-bisphosphate (PIP2) that is supported by a sub-membranous ßHeavy-Spectrin cytoskeleton. ßHeavy-spectrin mutant larvae affect ensheathing glial cell polarity with delocalized PIP2 and Nrv2 and exhibit an abnormal locomotion which is similarly shown by ensheathing glia ablated larvae. Thus, polarized glia compartmentalizes the brain and is essential for proper nervous system function.
dc.languageen
dc.publisherNature Publishing Group UK
dc.subjectArticle
dc.subject/631/378/87
dc.subject/631/378/1341
dc.subject/631/378/2596
dc.subject/64/24
dc.subject/38
dc.subject/38/1
dc.subject/38/89
dc.subject/38/35
dc.subjectarticle
dc.titleDrosophila ß Heavy -Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil
dc.typeArticle
dc.date.updated2022-01-31T16:21:09Z
prism.issueIdentifier1
prism.publicationNameNature Communications
prism.volume12
dc.identifier.doi10.17863/CAM.80892
dcterms.dateAccepted2021-10-06
rioxxterms.versionofrecord10.1038/s41467-021-26462-x
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidCardona, Albert [0000-0003-4941-6536]
dc.contributor.orcidKlämbt, Christian [0000-0002-6349-5800]
dc.identifier.eissn2041-1723


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