Drosophila ßHeavy-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil.

Authors
Pogodalla, Nicole 
Kranenburg, Holger 
Rey, Simone 
Rodrigues, Silke 

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Abstract

In the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute to the formation of an internal diffusion barrier. We find that ensheathing glia are polarized with a basolateral plasma membrane rich in phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and the Na+/K+-ATPase Nervana2 (Nrv2) that abuts an extracellular matrix formed at neuropil-cortex interface. The apical plasma membrane is facing the neuropil and is rich in phosphatidylinositol-(4,5)-bisphosphate (PIP2) that is supported by a sub-membranous ßHeavy-Spectrin cytoskeleton. ßHeavy-spectrin mutant larvae affect ensheathing glial cell polarity with delocalized PIP2 and Nrv2 and exhibit an abnormal locomotion which is similarly shown by ensheathing glia ablated larvae. Thus, polarized glia compartmentalizes the brain and is essential for proper nervous system function.

Publication Date
2021-11-04
Online Publication Date
2021-11-04
Acceptance Date
2021-10-06
Keywords
Animals, Brain, Cell Lineage, Drosophila, Drosophila Proteins, Glycoproteins, Larva, Nerve Tissue Proteins, Neuroglia, Neurons, Neuropil, Phosphatidylinositol Phosphates, Spectrin
Journal Title
Nat Commun
Journal ISSN
2041-1723
2041-1723
Volume Title
12
Publisher
Springer Science and Business Media LLC