Catalytic trajectory of a dimeric nonribosomal peptide synthetase subunit with an inserted epimerase domain.
Authors
Chen, Lu
Cao, Wei
Kong, Liangliang
Zhang, Wei
Publication Date
2022-02-01Journal Title
Nat Commun
ISSN
2041-1723
Publisher
Springer Science and Business Media LLC
Volume
13
Issue
1
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Wang, J., Li, D., Chen, L., Cao, W., Kong, L., Zhang, W., Croll, T., et al. (2022). Catalytic trajectory of a dimeric nonribosomal peptide synthetase subunit with an inserted epimerase domain.. Nat Commun, 13 (1) https://doi.org/10.1038/s41467-022-28284-x
Description
Funder: the National Science Foundation of China (32171252, 91753123, 31470830)
Abstract
Nonribosomal peptide synthetases (NRPSs) are modular assembly-line megaenzymes that synthesize diverse metabolites with wide-ranging biological activities. The structural dynamics of synthetic elongation has remained unclear. Here, we present cryo-EM structures of PchE, an NRPS elongation module, in distinct conformations. The domain organization reveals a unique "H"-shaped head-to-tail dimeric architecture. The capture of both aryl and peptidyl carrier protein-tethered substrates and intermediates inside the heterocyclization domain and L-cysteinyl adenylate in the adenylation domain illustrates the catalytic and recognition residues. The multilevel structural transitions guided by the adenylation C-terminal subdomain in combination with the inserted epimerase and the conformational changes of the heterocyclization tunnel are controlled by two residues. Moreover, we visualized the direct structural dynamics of the full catalytic cycle from thiolation to epimerization. This study establishes the catalytic trajectory of PchE and sheds light on the rational re-engineering of domain-inserted dimeric NRPSs for the production of novel pharmaceutical agents.
Keywords
Article, /631/535/1258/1259, /631/45/173, /101, /101/28, article
Sponsorship
Wellcome Trust (209407/Z/17/Z)
Identifiers
s41467-022-28284-x, 28284
External DOI: https://doi.org/10.1038/s41467-022-28284-x
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333506
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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