Implications of AlphaFold2 for crystallographic phasing by molecular replacement.
View / Open Files
Publication Date
2022-01-01Journal Title
Acta Crystallogr D Struct Biol
ISSN
2059-7983
Publisher
International Union of Crystallography (IUCr)
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
McCoy, A. J., Sammito, M. D., & Read, R. J. (2022). Implications of AlphaFold2 for crystallographic phasing by molecular replacement.. Acta Crystallogr D Struct Biol https://doi.org/10.1107/S2059798321012122
Abstract
The AlphaFold2 results in the 14th edition of Critical Assessment of Structure Prediction (CASP14) showed that accurate (low root-mean-square deviation) in silico models of protein structure domains are on the horizon, whether or not the protein is related to known structures through high-coverage sequence similarity. As highly accurate models become available, generated by harnessing the power of correlated mutations and deep learning, one of the aspects of structural biology to be impacted will be methods of phasing in crystallography. Here, the data from CASP14 are used to explore the prospects for changes in phasing methods, and in particular to explore the prospects for molecular-replacement phasing using in silico models.
Keywords
Molecular replacement, Protein Structure Prediction, Crystallographic Phase Problem, Alphafold2
Sponsorship
Wellcome Trust (209407/Z/17/Z)
Identifiers
34981757, PMC8725160
External DOI: https://doi.org/10.1107/S2059798321012122
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333679
Statistics
Total file downloads (since January 2020). For more information on metrics see the
IRUS guide.