An economic, square-shaped flat-field illumination module for TIRF-based super-resolution microscopy.
Lam, Jeff YL
Cheetham, Matthew R
Danial, John SH
Biophys Rep (N Y)
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Lam, J. Y., Wu, Y., Dimou, E., Zhang, Z., Cheetham, M. R., Körbel, M., Xia, Z., et al. (2022). An economic, square-shaped flat-field illumination module for TIRF-based super-resolution microscopy.. Biophys Rep (N Y), (100044), 100044-100044. https://doi.org/10.1016/j.bpr.2022.100044
Super-resolution microscopy allows complex biological assemblies to be observed with remarkable resolution. However, the presence of uneven Gaussian-shaped illumination hinders its use in quantitative imaging or high-throughput assays. Methods developed to circumvent this problem are often expensive, hard to implement, or not applicable to total internal reflection fluorescence imaging. We herein demonstrate a cost-effective method to overcome these challenges using a small square-core multimodal optical fiber as the coupler. We characterize our method with synthetic, recombinant, and cellular systems imaged under total internal reflection fluorescence and highly inclined and laminated optical sheet illuminations to demonstrate its ability to produce highly uniform images under all conditions.
UK Dementia Research Institute (PILOT2020-DANIAL)
European Research Council (669237)
External DOI: https://doi.org/10.1016/j.bpr.2022.100044
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333816
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