An economic, square-shaped flat-field illumination module for TIRF-based super-resolution microscopy.
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Authors
Lam, Jeff YL
Wu, Yunzhao
Dimou, Eleni
Zhang, Ziwei
Cheetham, Matthew R
Körbel, Markus
Xia, Zengjie
Danial, John SH
Publication Date
2022-03-09Journal Title
Biophys Rep (N Y)
ISSN
2667-0747
Publisher
Elsevier BV
Number
100044
Pages
100044-100044
Type
Article
This Version
AM
Metadata
Show full item recordCitation
Lam, J. Y., Wu, Y., Dimou, E., Zhang, Z., Cheetham, M. R., Körbel, M., Xia, Z., et al. (2022). An economic, square-shaped flat-field illumination module for TIRF-based super-resolution microscopy.. Biophys Rep (N Y), (100044), 100044-100044. https://doi.org/10.1016/j.bpr.2022.100044
Abstract
Super-resolution microscopy allows complex biological assemblies to be observed with remarkable resolution. However, the presence of uneven Gaussian-shaped illumination hinders its use in quantitative imaging or high-throughput assays. Methods developed to circumvent this problem are often expensive, hard to implement, or not applicable to total internal reflection fluorescence imaging. We herein demonstrate a cost-effective method to overcome these challenges using a small square-core multimodal optical fiber as the coupler. We characterize our method with synthetic, recombinant, and cellular systems imaged under total internal reflection fluorescence and highly inclined and laminated optical sheet illuminations to demonstrate its ability to produce highly uniform images under all conditions.
Sponsorship
UK Dementia Research Institute (MRC--)
European Research Council (669237)
Identifiers
External DOI: https://doi.org/10.1016/j.bpr.2022.100044
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333816
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