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dc.contributor.authorNeubauer, Anna
dc.contributor.authorRuaud, Stéphanie
dc.contributor.authorWaller, Manuel
dc.contributor.authorFrangedakis, Eftychios
dc.contributor.authorLi, Fay-Wei
dc.contributor.authorNötzold, Svenja I
dc.contributor.authorWicke, Susann
dc.contributor.authorBailly, Aurélien
dc.contributor.authorSzövényi, Péter
dc.date.accessioned2022-02-11T19:00:12Z
dc.date.available2022-02-11T19:00:12Z
dc.date.issued2022-03
dc.date.submitted2021-08-15
dc.identifier.issn2168-0450
dc.identifier.otheraps311456
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/333927
dc.description.abstractPremise: A detailed protocol for the protoplast transformation of hornwort tissue is not yet available, limiting molecular biological investigations of these plants and comparative analyses with other bryophytes, which display a gametophyte-dominant life cycle and are critical to understanding the evolution of key land plant traits. Methods and Results: We describe a detailed protocol to isolate and transiently transform protoplasts of the model hornwort Anthoceros agrestis. The digestion of liquid cultures with Driselase yields a high number of viable protoplasts suitable for polyethylene glycol (PEG)-mediated transformation. We also report early signs of protoplast regeneration, such as chloroplast division and cell wall reconstitution. Conclusions: This protocol represents a straightforward method for isolating and transforming A. agrestis protoplasts that is less laborious than previously described approaches. In combination with the recently developed stable genome transformation technique, this work further expands the prospects of functional studies in this model hornwort.
dc.languageen
dc.publisherWiley
dc.subjectPROTOCOL NOTE
dc.subjectAnthoceros
dc.subjecthornworts
dc.subjectmodel organism
dc.subjectprotoplasts
dc.subjecttransient transformation
dc.titleStep-by-step protocol for the isolation and transient transformation of hornwort protoplasts.
dc.typeArticle
dc.date.updated2022-02-11T19:00:12Z
prism.publicationNameAppl Plant Sci
dc.identifier.doi10.17863/CAM.81343
dcterms.dateAccepted2021-11-23
rioxxterms.versionofrecord10.1002/aps3.11456
rioxxterms.versionAO
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.contributor.orcidNeubauer, Anna [0000-0002-5102-2491]
dc.contributor.orcidRuaud, Stéphanie [0000-0001-7698-0605]
dc.contributor.orcidWaller, Manuel [0000-0002-6060-0740]
dc.contributor.orcidFrangedakis, Eftychios [0000-0002-3483-8464]
dc.contributor.orcidLi, Fay-Wei [0000-0002-0076-0152]
dc.contributor.orcidWicke, Susann [0000-0001-5785-9500]
dc.contributor.orcidBailly, Aurélien [0000-0001-5546-1945]
dc.contributor.orcidSzövényi, Péter [0000-0002-0324-4639]
dc.identifier.eissn2168-0450
cam.issuedOnline2022-02-11


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